Genetic analysis of liver neuraminidase isozymes in Rattus norvegicus: Independent control of NEU-1 and NEU-2 phenotypes

P. B. Samollow, J. L. Vandeberg, A. L. Ford, H. W. Kunz, T. J. Gill

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    5 Scopus citations

    Abstract

    Two recently identified isozymes of neuraminidase in rat liver were examined for transmission patterns and linkage relationships, and for variation among inbred strains. The isozymes, designated neuraminidase-1 (NEU-1) and neuraminidase-2 (NEU-2), exhibited no electrophoretic mobility variants among the 22 inbred strains examined, but did possess striking interstrain variation in activity phenotypes on electrophoretic gels. The results of a backcross analysis involving the KGH and ACP strains revealed that NEU-1 and NEU-2 phenotypes are independently controlled, each by a single autosomal locus with additively acting alleles. The two loci are unlinked to one another, but the gene controlling NEU-1 is tightly linked to RT1, the rat major histocompatibility complex. This gene is almost certainly identical to Neu-1, a gene identified previously through its effect on 'total' activity levels of liver neuraminidase as determined by fluorometric assay of tissue homogenates. NEU-2 and the gene controlling its phenotype were not detected by the fluorometric technique. We designate the genes controlling the NEU-1 and NEU-2 phenotypes as Neu-1 and Neu-2, respectively. Data from this and other studies place Neu-1 between Glo-1 and dw-3. The location of Neu-2 is unknown.

    Original languageEnglish (US)
    Pages (from-to)247-258
    Number of pages12
    JournalGenetics
    Volume114
    Issue number1
    StatePublished - Nov 26 1986

    ASJC Scopus subject areas

    • Genetics

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    Samollow, P. B., Vandeberg, J. L., Ford, A. L., Kunz, H. W., & Gill, T. J. (1986). Genetic analysis of liver neuraminidase isozymes in Rattus norvegicus: Independent control of NEU-1 and NEU-2 phenotypes. Genetics, 114(1), 247-258.