Genetic analysis of liver neuraminidase isozymes in Rattus norvegicus

Independent control of NEU-1 and NEU-2 phenotypes

P. B. Samollow, J. L. Vandeberg, A. L. Ford, H. W. Kunz, T. J. Gill

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Two recently identified isozymes of neuraminidase in rat liver were examined for transmission patterns and linkage relationships, and for variation among inbred strains. The isozymes, designated neuraminidase-1 (NEU-1) and neuraminidase-2 (NEU-2), exhibited no electrophoretic mobility variants among the 22 inbred strains examined, but did possess striking interstrain variation in activity phenotypes on electrophoretic gels. The results of a backcross analysis involving the KGH and ACP strains revealed that NEU-1 and NEU-2 phenotypes are independently controlled, each by a single autosomal locus with additively acting alleles. The two loci are unlinked to one another, but the gene controlling NEU-1 is tightly linked to RT1, the rat major histocompatibility complex. This gene is almost certainly identical to Neu-1, a gene identified previously through its effect on 'total' activity levels of liver neuraminidase as determined by fluorometric assay of tissue homogenates. NEU-2 and the gene controlling its phenotype were not detected by the fluorometric technique. We designate the genes controlling the NEU-1 and NEU-2 phenotypes as Neu-1 and Neu-2, respectively. Data from this and other studies place Neu-1 between Glo-1 and dw-3. The location of Neu-2 is unknown.

Original languageEnglish (US)
Pages (from-to)247-258
Number of pages12
JournalGenetics
Volume114
Issue number1
StatePublished - 1986
Externally publishedYes

Fingerprint

Neuraminidase
Isoenzymes
Phenotype
Liver
Genes
Major Histocompatibility Complex
Gels
Alleles

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Samollow, P. B., Vandeberg, J. L., Ford, A. L., Kunz, H. W., & Gill, T. J. (1986). Genetic analysis of liver neuraminidase isozymes in Rattus norvegicus: Independent control of NEU-1 and NEU-2 phenotypes. Genetics, 114(1), 247-258.

Genetic analysis of liver neuraminidase isozymes in Rattus norvegicus : Independent control of NEU-1 and NEU-2 phenotypes. / Samollow, P. B.; Vandeberg, J. L.; Ford, A. L.; Kunz, H. W.; Gill, T. J.

In: Genetics, Vol. 114, No. 1, 1986, p. 247-258.

Research output: Contribution to journalArticle

Samollow, PB, Vandeberg, JL, Ford, AL, Kunz, HW & Gill, TJ 1986, 'Genetic analysis of liver neuraminidase isozymes in Rattus norvegicus: Independent control of NEU-1 and NEU-2 phenotypes', Genetics, vol. 114, no. 1, pp. 247-258.
Samollow, P. B. ; Vandeberg, J. L. ; Ford, A. L. ; Kunz, H. W. ; Gill, T. J. / Genetic analysis of liver neuraminidase isozymes in Rattus norvegicus : Independent control of NEU-1 and NEU-2 phenotypes. In: Genetics. 1986 ; Vol. 114, No. 1. pp. 247-258.
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