Genetic ablation of the t-SNARE SNAP-25 distinguishes mechanisms of neuroexocytosis

Philip Washbourne; Peter M. Thompson; Mario Carta; Edmar T. Costa; James R. Mathews; Guillermina Lopez-Benditó; Zoltán Molnár; Mark W. Becher; C. Fernando Valenzuela; L. Donald Partridge; Michael C. Wilson

doi:10.1038/nn783

Genetic ablation of the t-SNARE SNAP-25 distinguishes mechanisms of neuroexocytosis

Philip Washbourne, Peter M. Thompson, Mario Carta, Edmar T. Costa, James R. Mathews, Guillermina Lopez-Benditó, Zoltán Molnár, Mark W. Becher, C. Fernando Valenzuela, L. Donald Partridge, Michael C. Wilson

Psychiatry

Research output: Contribution to journal › Article › peer-review

384 Scopus citations

Abstract

Axon outgrowth during development and neurotransmitter release depends on exocytotic mechanisms, although what protein machinery is common to or differentiates these processes remains unclear. Here we show that the neural t-SNARE (target-membrane-associated-soluble N-ethylmaleimide fusion protein attachment protein (SNAP) receptor) SNAP-25 is not required for nerve growth or stimulus-independent neurotransmitter release, but is essential for evoked synaptic transmission at neuromuscular junctions and central synapses. These results demonstrate that the development of neurotransmission requires the recruitment of a specialized SNARE core complex to meet the demands of regulated exocytosis.

Original language	English (US)
Pages (from-to)	19-26
Number of pages	8
Journal	Nature Neuroscience
Volume	5
Issue number	1
DOIs	https://doi.org/10.1038/nn783
State	Published - Jan 2002

ASJC Scopus subject areas

Neuroscience(all)

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Genetic ablation of the t-SNARE SNAP-25 distinguishes mechanisms of neuroexocytosis. / Washbourne, Philip; Thompson, Peter M.; Carta, Mario; Costa, Edmar T.; Mathews, James R.; Lopez-Benditó, Guillermina; Molnár, Zoltán; Becher, Mark W.; Valenzuela, C. Fernando; Partridge, L. Donald; Wilson, Michael C.

In: Nature Neuroscience, Vol. 5, No. 1, 01.2002, p. 19-26.

Research output: Contribution to journal › Article › peer-review

Washbourne, Philip ; Thompson, Peter M. ; Carta, Mario ; Costa, Edmar T. ; Mathews, James R. ; Lopez-Benditó, Guillermina ; Molnár, Zoltán ; Becher, Mark W. ; Valenzuela, C. Fernando ; Partridge, L. Donald ; Wilson, Michael C. / Genetic ablation of the t-SNARE SNAP-25 distinguishes mechanisms of neuroexocytosis. In: Nature Neuroscience. 2002 ; Vol. 5, No. 1. pp. 19-26.

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