Genes flanking Xist in mouse and human are separated on the X chromosome in American marsupials

Alexander I. Shevchenko, Irina S. Zakharova, Eugeny A. Elisaphenko, Nicolay N. Kolesnikov, Siobhan Whitehead, Christine Bird, Mark Ross, Jennifer R. Weidman, Randy L. Jirtle, Tatiana V. Karamysheva, Nicolay B. Rubtsov, John L. VandeBerg, Nina A. Mazurok, Tatyana B. Nesterova, Neil Brockdorff, Suren M. Zakian

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

X inactivation, the transcriptional silencing of one of the two X chromosomes in female mammals, achieves dosage compensation of X-linked genes relative to XY males. In eutherian mammals X inactivation is regulated by the X-inactive specific transcript (Xist), a cis-acting non-coding RNA that triggers silencing of the chromosome from which it is transcribed. Marsupial mammals also undergo X inactivation but the mechanism is relatively poorly understood. We set out to analyse the X chromosome in Monodelphis domestica and Didelphis virginiana, focusing on characterizing the interval defined by the Chic1 and Slc16a2 genes that in eutherians flank the Xist locus. The synteny of this region is retained on chicken chromosome 4 where other loci belonging to the evolutionarily ancient stratum of the human X chromosome, the so-called X conserved region (XCR), are also located. We show that in both M. domestica and D. virginiana an evolutionary breakpoint has separated the Chic1 and Slc16a2 loci. Detailed analysis of opossum genomic sequences revealed linkage of Chic1 with the Lnx3 gene, recently proposed to be the evolutionary precursor of Xist, and Fip1, the evolutionary precursor of Tsx, a gene located immediately downstream of Xist in eutherians. We discuss these findings in relation to the evolution of Xist and X inactivation in mammals.

Original languageEnglish (US)
Pages (from-to)127-136
Number of pages10
JournalChromosome Research
Volume15
Issue number2
DOIs
StatePublished - Feb 2007
Externally publishedYes

Fingerprint

Marsupialia
X Chromosome
X Chromosome Inactivation
Mammals
Monodelphis
Didelphis
Genes
Chromosomes, Human, X
Synteny
Opossums
X-Linked Genes
Untranslated RNA
Chromosomes, Human, Pair 4
RNA Interference
Chickens
Chromosomes
XIST non-coding RNA

Keywords

  • Didelphis virginiana
  • Marsupial
  • Monodelphis domestica
  • Opossum
  • X chromosome
  • X inactivation
  • Xist

ASJC Scopus subject areas

  • Genetics

Cite this

Shevchenko, A. I., Zakharova, I. S., Elisaphenko, E. A., Kolesnikov, N. N., Whitehead, S., Bird, C., ... Zakian, S. M. (2007). Genes flanking Xist in mouse and human are separated on the X chromosome in American marsupials. Chromosome Research, 15(2), 127-136. https://doi.org/10.1007/s10577-006-1115-9

Genes flanking Xist in mouse and human are separated on the X chromosome in American marsupials. / Shevchenko, Alexander I.; Zakharova, Irina S.; Elisaphenko, Eugeny A.; Kolesnikov, Nicolay N.; Whitehead, Siobhan; Bird, Christine; Ross, Mark; Weidman, Jennifer R.; Jirtle, Randy L.; Karamysheva, Tatiana V.; Rubtsov, Nicolay B.; VandeBerg, John L.; Mazurok, Nina A.; Nesterova, Tatyana B.; Brockdorff, Neil; Zakian, Suren M.

In: Chromosome Research, Vol. 15, No. 2, 02.2007, p. 127-136.

Research output: Contribution to journalArticle

Shevchenko, AI, Zakharova, IS, Elisaphenko, EA, Kolesnikov, NN, Whitehead, S, Bird, C, Ross, M, Weidman, JR, Jirtle, RL, Karamysheva, TV, Rubtsov, NB, VandeBerg, JL, Mazurok, NA, Nesterova, TB, Brockdorff, N & Zakian, SM 2007, 'Genes flanking Xist in mouse and human are separated on the X chromosome in American marsupials', Chromosome Research, vol. 15, no. 2, pp. 127-136. https://doi.org/10.1007/s10577-006-1115-9
Shevchenko AI, Zakharova IS, Elisaphenko EA, Kolesnikov NN, Whitehead S, Bird C et al. Genes flanking Xist in mouse and human are separated on the X chromosome in American marsupials. Chromosome Research. 2007 Feb;15(2):127-136. https://doi.org/10.1007/s10577-006-1115-9
Shevchenko, Alexander I. ; Zakharova, Irina S. ; Elisaphenko, Eugeny A. ; Kolesnikov, Nicolay N. ; Whitehead, Siobhan ; Bird, Christine ; Ross, Mark ; Weidman, Jennifer R. ; Jirtle, Randy L. ; Karamysheva, Tatiana V. ; Rubtsov, Nicolay B. ; VandeBerg, John L. ; Mazurok, Nina A. ; Nesterova, Tatyana B. ; Brockdorff, Neil ; Zakian, Suren M. / Genes flanking Xist in mouse and human are separated on the X chromosome in American marsupials. In: Chromosome Research. 2007 ; Vol. 15, No. 2. pp. 127-136.
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AU - Bird, Christine

AU - Ross, Mark

AU - Weidman, Jennifer R.

AU - Jirtle, Randy L.

AU - Karamysheva, Tatiana V.

AU - Rubtsov, Nicolay B.

AU - VandeBerg, John L.

AU - Mazurok, Nina A.

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AU - Brockdorff, Neil

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