Genes flanking Xist in mouse and human are separated on the X chromosome in American marsupials

Alexander I. Shevchenko, Irina S. Zakharova, Eugeny A. Elisaphenko, Nicolay N. Kolesnikov, Siobhan Whitehead, Christine Bird, Mark Ross, Jennifer R. Weidman, Randy L. Jirtle, Tatiana V. Karamysheva, Nicolay B. Rubtsov, John L. VandeBerg, Nina A. Mazurok, Tatyana B. Nesterova, Neil Brockdorff, Suren M. Zakian

    Research output: Contribution to journalArticlepeer-review

    42 Scopus citations

    Abstract

    X inactivation, the transcriptional silencing of one of the two X chromosomes in female mammals, achieves dosage compensation of X-linked genes relative to XY males. In eutherian mammals X inactivation is regulated by the X-inactive specific transcript (Xist), a cis-acting non-coding RNA that triggers silencing of the chromosome from which it is transcribed. Marsupial mammals also undergo X inactivation but the mechanism is relatively poorly understood. We set out to analyse the X chromosome in Monodelphis domestica and Didelphis virginiana, focusing on characterizing the interval defined by the Chic1 and Slc16a2 genes that in eutherians flank the Xist locus. The synteny of this region is retained on chicken chromosome 4 where other loci belonging to the evolutionarily ancient stratum of the human X chromosome, the so-called X conserved region (XCR), are also located. We show that in both M. domestica and D. virginiana an evolutionary breakpoint has separated the Chic1 and Slc16a2 loci. Detailed analysis of opossum genomic sequences revealed linkage of Chic1 with the Lnx3 gene, recently proposed to be the evolutionary precursor of Xist, and Fip1, the evolutionary precursor of Tsx, a gene located immediately downstream of Xist in eutherians. We discuss these findings in relation to the evolution of Xist and X inactivation in mammals.

    Original languageEnglish (US)
    Pages (from-to)127-136
    Number of pages10
    JournalChromosome Research
    Volume15
    Issue number2
    DOIs
    StatePublished - Feb 2007

    Keywords

    • Didelphis virginiana
    • Marsupial
    • Monodelphis domestica
    • Opossum
    • X chromosome
    • X inactivation
    • Xist

    ASJC Scopus subject areas

    • Genetics

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