Generation of site-appropriate tissue by a living cellular sheet in the treatment of mucogingival defects

E. Todd Scheyer, Marc L. Nevins, Rodrigo Neiva, David L. Cochran, William V. Giannobile, Sook Bin Woo, William N. King, John K. Spitznagel, Damien Bates, Michael K. McGuire

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


Generation of site-appropriate tissue in the oral cavity includes the restoration of the correct anatomic type, amount, and distribution of the tissue. This study is a post hoc analysis of data collected during previously published results from two randomized clinical trials of a living cellular sheet (LCS; allogenic cultured keratinocytes and fibroblasts in bovine collagen) versus a free gingival graft (FGG), evaluating their ability to augment keratinized tissue or gingiva. Methods: Post hoc histologic and clinical (photographic) comparisons of the outcomes of treatment were performed on histologic and photographic data gathered in the two randomized clinical trials. Results: Histologic findings showed that LCS-treated sites resembled gingiva rather than alveolar mucosa. Photographic analysis indicated that LCS treatment resulted in more site-appropriate tissue than FGG in terms of tissue color, with adjacent untreated tissue, absence of scar formation or keloid-like appearance, and mucogingival junction alignment. Conclusion: Treatment of mucogingival defects with LCS resulted in the generation of tissue that is more site appropriate than tissue transplanted from the palate. J Periodontol 2014;85:e57-e64.

Original languageEnglish (US)
Pages (from-to)e57-e64
JournalJournal of periodontology
Issue number4
StatePublished - Apr 2014


  • Gingival recession
  • Periodontics
  • Regenerative medicine
  • Tissue engineering

ASJC Scopus subject areas

  • Periodontics


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