Generation of recombinant extracellular fragment of vascular endothelial growth factor receptor 2 and specific monoclonal antibodies to this receptor

A. A. Korchagina; S. A. Shein; A. V. Leopol'D; N. E. Volgina; O. I. Gurina; I. P. Lazarenko; O. M. Antonova; V. P. Baklaushev; V. P. Chekhonin

doi:10.1007/s10517-014-2348-9

Generation of recombinant extracellular fragment of vascular endothelial growth factor receptor 2 and specific monoclonal antibodies to this receptor

A. A. Korchagina, S. A. Shein, A. V. Leopol'D, N. E. Volgina, O. I. Gurina, I. P. Lazarenko, O. M. Antonova, V. P. Baklaushev, V. P. Chekhonin

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

cDNA extracellular Ig-like domains I-III of vascular endothelial growth factor receptor 2 (VEGFR2) was cloned in an expressing vector pET-32a. Western blotting showed immunochemical identity of recombinant VEGFR2_I-III produced by prokaryotic expression system to the native receptor. BALB/c mice were immunized with VEGFR2_I-III for obtaining specific antibodies to VEGFR2. Hybridomas producing monoclonal antibodies were selected by ELISA, Western blotting, and immunocytochemical assay. Thus, we obtained hybridoma producing monoclonal antibodies to VEGFR2 that selectively interact with both recombinant and native extracellular fragment of the receptor.

Original language	English (US)
Pages (from-to)	357-362
Number of pages	6
Journal	Bulletin of Experimental Biology and Medicine
Volume	156
Issue number	3
DOIs	https://doi.org/10.1007/s10517-014-2348-9
State	Published - Jan 2014
Externally published	Yes

Keywords

competitive interaction
monoclonal antibodies
vascular endothelial growth factor

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1007/s10517-014-2348-9

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Korchagina, A. A., Shein, S. A., Leopol'D, A. V., Volgina, N. E., Gurina, O. I., Lazarenko, I. P., Antonova, O. M., Baklaushev, V. P., & Chekhonin, V. P. (2014). Generation of recombinant extracellular fragment of vascular endothelial growth factor receptor 2 and specific monoclonal antibodies to this receptor. Bulletin of Experimental Biology and Medicine, 156(3), 357-362. https://doi.org/10.1007/s10517-014-2348-9

Korchagina, AA , Shein, SA, Leopol'D, AV, Volgina, NE, Gurina, OI, Lazarenko, IP, Antonova, OM, Baklaushev, VP & Chekhonin, VP 2014, 'Generation of recombinant extracellular fragment of vascular endothelial growth factor receptor 2 and specific monoclonal antibodies to this receptor', Bulletin of Experimental Biology and Medicine, vol. 156, no. 3, pp. 357-362. https://doi.org/10.1007/s10517-014-2348-9

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abstract = "cDNA extracellular Ig-like domains I-III of vascular endothelial growth factor receptor 2 (VEGFR2) was cloned in an expressing vector pET-32a. Western blotting showed immunochemical identity of recombinant VEGFR2I-III produced by prokaryotic expression system to the native receptor. BALB/c mice were immunized with VEGFR2I-III for obtaining specific antibodies to VEGFR2. Hybridomas producing monoclonal antibodies were selected by ELISA, Western blotting, and immunocytochemical assay. Thus, we obtained hybridoma producing monoclonal antibodies to VEGFR2 that selectively interact with both recombinant and native extracellular fragment of the receptor.",

keywords = "competitive interaction, monoclonal antibodies, vascular endothelial growth factor",

author = "Korchagina, {A. A.} and Shein, {S. A.} and Leopol'D, {A. V.} and Volgina, {N. E.} and Gurina, {O. I.} and Lazarenko, {I. P.} and Antonova, {O. M.} and Baklaushev, {V. P.} and Chekhonin, {V. P.}",

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doi = "10.1007/s10517-014-2348-9",

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T1 - Generation of recombinant extracellular fragment of vascular endothelial growth factor receptor 2 and specific monoclonal antibodies to this receptor

AU - Korchagina, A. A.

AU - Shein, S. A.

AU - Leopol'D, A. V.

AU - Volgina, N. E.

AU - Gurina, O. I.

AU - Lazarenko, I. P.

AU - Antonova, O. M.

AU - Baklaushev, V. P.

AU - Chekhonin, V. P.

PY - 2014/1

Y1 - 2014/1

N2 - cDNA extracellular Ig-like domains I-III of vascular endothelial growth factor receptor 2 (VEGFR2) was cloned in an expressing vector pET-32a. Western blotting showed immunochemical identity of recombinant VEGFR2I-III produced by prokaryotic expression system to the native receptor. BALB/c mice were immunized with VEGFR2I-III for obtaining specific antibodies to VEGFR2. Hybridomas producing monoclonal antibodies were selected by ELISA, Western blotting, and immunocytochemical assay. Thus, we obtained hybridoma producing monoclonal antibodies to VEGFR2 that selectively interact with both recombinant and native extracellular fragment of the receptor.

AB - cDNA extracellular Ig-like domains I-III of vascular endothelial growth factor receptor 2 (VEGFR2) was cloned in an expressing vector pET-32a. Western blotting showed immunochemical identity of recombinant VEGFR2I-III produced by prokaryotic expression system to the native receptor. BALB/c mice were immunized with VEGFR2I-III for obtaining specific antibodies to VEGFR2. Hybridomas producing monoclonal antibodies were selected by ELISA, Western blotting, and immunocytochemical assay. Thus, we obtained hybridoma producing monoclonal antibodies to VEGFR2 that selectively interact with both recombinant and native extracellular fragment of the receptor.

KW - competitive interaction

KW - monoclonal antibodies

KW - vascular endothelial growth factor

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Generation of recombinant extracellular fragment of vascular endothelial growth factor receptor 2 and specific monoclonal antibodies to this receptor

Abstract

Keywords

ASJC Scopus subject areas

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