Generation of monoclonal antibodies to recombinant vascular endothelial growth factor

S. A. Shein; O. I. Gurina; A. V. Leopol'D; V. P. Baklaushev; A. A. Korchagina; N. F. Grinenko; N. V. Ivanova; N. E. Volgina; I. A. Ryabukhin; V. P. Chekhonin

doi:10.1007/s10517-012-1662-3

Generation of monoclonal antibodies to recombinant vascular endothelial growth factor

S. A. Shein
, O. I. Gurina
, A. V. Leopol'D
, V. P. Baklaushev
, A. A. Korchagina
, N. F. Grinenko
, N. V. Ivanova
, N. E. Volgina
, I. A. Ryabukhin
, V. P. Chekhonin

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Female BALB/c mice were subcutaneously immunized with recombinant VEGF-164. After 3 immunization cycles, splenic B cells from immunized mouse were fused with immortalized myeloma culture SP2/0-Ag14 cells. Screening of hybrid cells producing anti-VEGF antibodies was performed by ELISA and immunocytochemical analysis on cultured C6 glioma cells. Subsequent cloning yielded hybridoma stably expressing monoclonal anti-VEGF antibodies recognizing recombinant and native VEGF.

Original language	English (US)
Pages (from-to)	139-142
Number of pages	4
Journal	Bulletin of Experimental Biology and Medicine
Volume	153
Issue number	1
DOIs	https://doi.org/10.1007/s10517-012-1662-3
State	Published - May 2012
Externally published	Yes

Keywords

monoclonal antibodies, anti-VEGF antibodies
vascular endothelial growth factor

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1007/s10517-012-1662-3

Cite this

Shein, S. A., Gurina, O. I., Leopol'D, A. V., Baklaushev, V. P., Korchagina, A. A., Grinenko, N. F., Ivanova, N. V., Volgina, N. E., Ryabukhin, I. A., & Chekhonin, V. P. (2012). Generation of monoclonal antibodies to recombinant vascular endothelial growth factor. Bulletin of Experimental Biology and Medicine, 153(1), 139-142. https://doi.org/10.1007/s10517-012-1662-3

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title = "Generation of monoclonal antibodies to recombinant vascular endothelial growth factor",

abstract = "Female BALB/c mice were subcutaneously immunized with recombinant VEGF-164. After 3 immunization cycles, splenic B cells from immunized mouse were fused with immortalized myeloma culture SP2/0-Ag14 cells. Screening of hybrid cells producing anti-VEGF antibodies was performed by ELISA and immunocytochemical analysis on cultured C6 glioma cells. Subsequent cloning yielded hybridoma stably expressing monoclonal anti-VEGF antibodies recognizing recombinant and native VEGF.",

keywords = "monoclonal antibodies, anti-VEGF antibodies, vascular endothelial growth factor",

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year = "2012",

month = may,

doi = "10.1007/s10517-012-1662-3",

language = "English (US)",

volume = "153",

pages = "139--142",

journal = "Bulletin of Experimental Biology and Medicine",

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TY - JOUR

T1 - Generation of monoclonal antibodies to recombinant vascular endothelial growth factor

AU - Shein, S. A.

AU - Gurina, O. I.

AU - Leopol'D, A. V.

AU - Baklaushev, V. P.

AU - Korchagina, A. A.

AU - Grinenko, N. F.

AU - Ivanova, N. V.

AU - Volgina, N. E.

AU - Ryabukhin, I. A.

AU - Chekhonin, V. P.

PY - 2012/5

Y1 - 2012/5

N2 - Female BALB/c mice were subcutaneously immunized with recombinant VEGF-164. After 3 immunization cycles, splenic B cells from immunized mouse were fused with immortalized myeloma culture SP2/0-Ag14 cells. Screening of hybrid cells producing anti-VEGF antibodies was performed by ELISA and immunocytochemical analysis on cultured C6 glioma cells. Subsequent cloning yielded hybridoma stably expressing monoclonal anti-VEGF antibodies recognizing recombinant and native VEGF.

AB - Female BALB/c mice were subcutaneously immunized with recombinant VEGF-164. After 3 immunization cycles, splenic B cells from immunized mouse were fused with immortalized myeloma culture SP2/0-Ag14 cells. Screening of hybrid cells producing anti-VEGF antibodies was performed by ELISA and immunocytochemical analysis on cultured C6 glioma cells. Subsequent cloning yielded hybridoma stably expressing monoclonal anti-VEGF antibodies recognizing recombinant and native VEGF.

KW - monoclonal antibodies, anti-VEGF antibodies

KW - vascular endothelial growth factor

UR - https://www.scopus.com/pages/publications/84862771986

UR - https://www.scopus.com/pages/publications/84862771986#tab=citedBy

U2 - 10.1007/s10517-012-1662-3

DO - 10.1007/s10517-012-1662-3

M3 - Article

C2 - 22808513

AN - SCOPUS:84862771986

SN - 0007-4888

VL - 153

SP - 139

EP - 142

JO - Bulletin of Experimental Biology and Medicine

JF - Bulletin of Experimental Biology and Medicine

IS - 1

ER -

Generation of monoclonal antibodies to recombinant vascular endothelial growth factor

Abstract

Keywords

ASJC Scopus subject areas

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