Abstract
Female BALB/c mice were subcutaneously immunized with recombinant VEGF-164. After 3 immunization cycles, splenic B cells from immunized mouse were fused with immortalized myeloma culture SP2/0-Ag14 cells. Screening of hybrid cells producing anti-VEGF antibodies was performed by ELISA and immunocytochemical analysis on cultured C6 glioma cells. Subsequent cloning yielded hybridoma stably expressing monoclonal anti-VEGF antibodies recognizing recombinant and native VEGF.
Original language | English (US) |
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Pages (from-to) | 139-142 |
Number of pages | 4 |
Journal | Bulletin of Experimental Biology and Medicine |
Volume | 153 |
Issue number | 1 |
DOIs | |
State | Published - May 2012 |
Externally published | Yes |
Keywords
- monoclonal antibodies, anti-VEGF antibodies
- vascular endothelial growth factor
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology