Generation of monoclonal antibodies to recombinant vascular endothelial growth factor

S. A. Shein, O. I. Gurina, A. V. Leopol'D, V. P. Baklaushev, A. A. Korchagina, N. F. Grinenko, N. V. Ivanova, N. E. Volgina, I. A. Ryabukhin, V. P. Chekhonin

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Female BALB/c mice were subcutaneously immunized with recombinant VEGF-164. After 3 immunization cycles, splenic B cells from immunized mouse were fused with immortalized myeloma culture SP2/0-Ag14 cells. Screening of hybrid cells producing anti-VEGF antibodies was performed by ELISA and immunocytochemical analysis on cultured C6 glioma cells. Subsequent cloning yielded hybridoma stably expressing monoclonal anti-VEGF antibodies recognizing recombinant and native VEGF.

Original languageEnglish (US)
Pages (from-to)139-142
Number of pages4
JournalBulletin of Experimental Biology and Medicine
Volume153
Issue number1
DOIs
StatePublished - May 2012
Externally publishedYes

Keywords

  • monoclonal antibodies, anti-VEGF antibodies
  • vascular endothelial growth factor

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

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