Generation of an integration-free iPSC line, ICCSICi006-A, derived from a male Alzheimer's disease patient carrying the PSEN1-G206D mutation

Eva Díaz-Guerra; Manuel A. Oria-Muriel; Elena P. Moreno-Jiménez; Itziar de Rojas; César Rodríguez; Eva Rodríguez-Traver; María Orera; Isabel Hernández; Agustín Ruiz; Carlos Vicario

doi:10.1016/j.scr.2019.101574

Generation of an integration-free iPSC line, ICCSICi006-A, derived from a male Alzheimer's disease patient carrying the PSEN1-G206D mutation

Eva Díaz-Guerra, Manuel A. Oria-Muriel, Elena P. Moreno-Jiménez, Itziar de Rojas, César Rodríguez, Eva Rodríguez-Traver, María Orera, Isabel Hernández, Agustín Ruiz, Carlos Vicario

Research output: Contribution to journal › Article › peer-review

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Abstract

The familial form of Alzheimer's disease (FAD), which is caused by mutations in PRESENILIN 1 (PSEN1) and amyloid precursor protein (APP) genes, represents less than 5% of all AD cases and has an early-onset. We report the generation and characterization of an iPSC line derived from a FAD patient carrying the PSEN1-G206D mutation. The iPSC line maintained the original genotype, a normal karyotype, was free from Sendai viral vectors and reprogramming factors (OCT4, SOX2, KLF4 and c-MYC), presented a typical morphology, expressed endogenous pluripotency markers, and could be differentiated into ectodermal, mesodermal and endodermal cells, confirming its pluripotency.

Original language	English (US)
Article number	101574
Journal	Stem Cell Research
Volume	40
DOIs	https://doi.org/10.1016/j.scr.2019.101574
State	Published - Oct 2019
Externally published	Yes

ASJC Scopus subject areas

Developmental Biology
Cell Biology

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Díaz-Guerra, E., Oria-Muriel, M. A., Moreno-Jiménez, E. P., de Rojas, I., Rodríguez, C., Rodríguez-Traver, E., Orera, M., Hernández, I., Ruiz, A., & Vicario, C. (2019). Generation of an integration-free iPSC line, ICCSICi006-A, derived from a male Alzheimer's disease patient carrying the PSEN1-G206D mutation. Stem Cell Research, 40, Article 101574. https://doi.org/10.1016/j.scr.2019.101574

Díaz-Guerra, E, Oria-Muriel, MA, Moreno-Jiménez, EP, de Rojas, I, Rodríguez, C, Rodríguez-Traver, E, Orera, M, Hernández, I, Ruiz, A & Vicario, C 2019, 'Generation of an integration-free iPSC line, ICCSICi006-A, derived from a male Alzheimer's disease patient carrying the PSEN1-G206D mutation', Stem Cell Research, vol. 40, 101574. https://doi.org/10.1016/j.scr.2019.101574

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title = "Generation of an integration-free iPSC line, ICCSICi006-A, derived from a male Alzheimer's disease patient carrying the PSEN1-G206D mutation",

abstract = "The familial form of Alzheimer's disease (FAD), which is caused by mutations in PRESENILIN 1 (PSEN1) and amyloid precursor protein (APP) genes, represents less than 5% of all AD cases and has an early-onset. We report the generation and characterization of an iPSC line derived from a FAD patient carrying the PSEN1-G206D mutation. The iPSC line maintained the original genotype, a normal karyotype, was free from Sendai viral vectors and reprogramming factors (OCT4, SOX2, KLF4 and c-MYC), presented a typical morphology, expressed endogenous pluripotency markers, and could be differentiated into ectodermal, mesodermal and endodermal cells, confirming its pluripotency.",

author = "Eva D{\'i}az-Guerra and Oria-Muriel, {Manuel A.} and Moreno-Jim{\'e}nez, {Elena P.} and {de Rojas}, Itziar and C{\'e}sar Rodr{\'i}guez and Eva Rodr{\'i}guez-Traver and Mar{\'i}a Orera and Isabel Hern{\'a}ndez and Agust{\'i}n Ruiz and Carlos Vicario",

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year = "2019",

month = oct,

doi = "10.1016/j.scr.2019.101574",

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journal = "Stem Cell Research",

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AU - Oria-Muriel, Manuel A.

AU - Moreno-Jiménez, Elena P.

AU - de Rojas, Itziar

AU - Rodríguez, César

AU - Rodríguez-Traver, Eva

AU - Orera, María

AU - Hernández, Isabel

AU - Ruiz, Agustín

AU - Vicario, Carlos

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AB - The familial form of Alzheimer's disease (FAD), which is caused by mutations in PRESENILIN 1 (PSEN1) and amyloid precursor protein (APP) genes, represents less than 5% of all AD cases and has an early-onset. We report the generation and characterization of an iPSC line derived from a FAD patient carrying the PSEN1-G206D mutation. The iPSC line maintained the original genotype, a normal karyotype, was free from Sendai viral vectors and reprogramming factors (OCT4, SOX2, KLF4 and c-MYC), presented a typical morphology, expressed endogenous pluripotency markers, and could be differentiated into ectodermal, mesodermal and endodermal cells, confirming its pluripotency.

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Generation of an integration-free iPSC line, ICCSICi006-A, derived from a male Alzheimer's disease patient carrying the PSEN1-G206D mutation

Abstract

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