Generation of alloreactive cytolytic T lymphocytes by immobilized anti-CD3 monoclonal antibodies. Analysis of requirements for human cytolytic T-lymphocyte differentiation

R. De Jong, M. Brouwer, V. I. Rebel, G. A. Van Seventer, F. Miedema, R. A W Van Lier

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Requirements for the induction of human cytolytic T-lymphocyte (CTL) activity were studied in a monocyte-free T-cell activation system that uses immobilized anti-CD3 monoclonal antibodies (mAb) as a stimulus. Alloreactive CTL with specificity for HLA-A and -B locus antigens could be demonstrated within 2 days after the initiation of activation. CTL induction in purified T cells initiated by an optimal concentration of immobilized anti-CD3 mAb was not enhanced by the addition of monocytes or exogeneous cytokines, whereas addition of anti-CD25 mAb largely blocked the response. Upon suboptimal anti-CD3 mAb stimulation, addition of recombinant interleukin (rIL)-2, rIL-1 and rIL-4, but not recombinant interferon-gamma (IFN-γ) or rIL-6, potentiated the development of CTL activity. Finally it was shown that immobilized anti-CD3 mAb induced significant levels of CTL activity in both purified CD4+ and CD8+ cells. This study indicates that the requirement for cytokines in the differentiation of CTL precursors depends on the strength of the activation signal delivered through the T-cell receptor.

Original languageEnglish (US)
Pages (from-to)357-364
Number of pages8
JournalImmunology
Volume70
Issue number3
StatePublished - 1990
Externally publishedYes

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Monoclonal Antibodies
T-Lymphocytes
Monocytes
Cytokines
HLA-A Antigens
HLA-B Antigens
T-Cell Antigen Receptor
Interleukin-1
Interleukin-4
Interferon-gamma
Interleukin-2
Interleukin-6
Antigens

ASJC Scopus subject areas

  • Immunology

Cite this

De Jong, R., Brouwer, M., Rebel, V. I., Van Seventer, G. A., Miedema, F., & Van Lier, R. A. W. (1990). Generation of alloreactive cytolytic T lymphocytes by immobilized anti-CD3 monoclonal antibodies. Analysis of requirements for human cytolytic T-lymphocyte differentiation. Immunology, 70(3), 357-364.

Generation of alloreactive cytolytic T lymphocytes by immobilized anti-CD3 monoclonal antibodies. Analysis of requirements for human cytolytic T-lymphocyte differentiation. / De Jong, R.; Brouwer, M.; Rebel, V. I.; Van Seventer, G. A.; Miedema, F.; Van Lier, R. A W.

In: Immunology, Vol. 70, No. 3, 1990, p. 357-364.

Research output: Contribution to journalArticle

De Jong, R. ; Brouwer, M. ; Rebel, V. I. ; Van Seventer, G. A. ; Miedema, F. ; Van Lier, R. A W. / Generation of alloreactive cytolytic T lymphocytes by immobilized anti-CD3 monoclonal antibodies. Analysis of requirements for human cytolytic T-lymphocyte differentiation. In: Immunology. 1990 ; Vol. 70, No. 3. pp. 357-364.
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AU - Van Seventer, G. A.

AU - Miedema, F.

AU - Van Lier, R. A W

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