Generation of a consensus sequence from prevalent and incident HIV-1 infections in West Africa to guide AIDS vaccine development

Dennis L. Ellenberger, Bin Li, L. Davis Lupo, S. Michele Owen, John Nkengasong, Madeleine Sassan Kadio-Morokro, James Smith, Harriet Robinson, Marta Ackers, Alan Greenberg, Thomas Folks, Salvatore Butera

    Research output: Contribution to journalArticlepeer-review

    42 Scopus citations

    Abstract

    We considered several key issues regarding the development of a DNA-based human immunodeficiency virus type 1 (HIV-1) vaccine: (1) should the candidate vaccine construct be derived from incident or prevalent HIV-1 strains; and (2) should circulating plasma virus, archived HIV-1 provirus recovered from peripheral blood mononuclear cells, or both be included? To address these questions, we collected circulating HIV-1 strains from infected individuals residing in Abidjan, Côte d'Ivoire. From a panel of 23 strains, 22 were HIV-1 subtype A in gag, 19 of which phylogenetically clustered with the recombinant HIV-1, CRF02-AG strains from West Africa. The mosaic genome of CRF02-AG was confirmed by sequencing the protease gene. A consensus gag p24 protein sequence was generated and 147 of 148 codons were identical to CRF02-AG (IbNG). Regardless of the sequence origin (RNA, provirus, incident, or prevalent), the gag p24 consensus sequences were highly representative of these distinct virologic compartments. These data suggest that the consensus sequence generated from incident and prevalent infections may provide an appropriate sequence for a DNA vaccine and is largely representative of the major circulating viral strain.

    Original languageEnglish (US)
    Pages (from-to)155-163
    Number of pages9
    JournalVirology
    Volume302
    Issue number1
    DOIs
    StatePublished - 2002

    Keywords

    • CRF02-AG
    • Consensus sequence
    • Côte d'Ivoire
    • HIV-1 vaccine design
    • Virologic compartments

    ASJC Scopus subject areas

    • Virology

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