Generation and characterization of monoclonal antibodies specific for vaccinia virus.

Xiangzhi Meng, Yan Xiang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Monoclonal antibodies to specific vaccinia virus (VACV) proteins are valuable reagents in studies of VACV. In this chapter, we describe methods of generating a panel of monoclonal antibodies that recognize a variety of VACV proteins in their native conformation in infected cells. The antibodies thus generated recognize mostly VACV proteins that are involved in virion assembly or/and are major antigens in smallpox vaccine. These antibodies are useful for tracking distinct steps in virion assembly and for studying the B cell epitopes in smallpox vaccine.

Original languageEnglish (US)
Pages (from-to)219-232
Number of pages14
JournalMethods in molecular biology (Clifton, N.J.)
Volume890
DOIs
StatePublished - 2012

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Vaccinia virus
Monoclonal Antibodies
Smallpox Vaccine
Virion
B-Lymphocyte Epitopes
Proteins
Antibodies
Antigens

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Generation and characterization of monoclonal antibodies specific for vaccinia virus. / Meng, Xiangzhi; Xiang, Yan.

In: Methods in molecular biology (Clifton, N.J.), Vol. 890, 2012, p. 219-232.

Research output: Contribution to journalArticle

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