Generation and characterization of monoclonal antibodies specific for vaccinia virus

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Monoclonal antibodies to specific vaccinia virus (VACV) proteins are valuable reagents in studies of VACV. In this chapter, we describe methods of generating a panel of monoclonal antibodies that recognize a variety of VACV proteins in their native conformation in infected cells. The antibodies thus generated recognize mostly VACV proteins that are involved in virion assembly or/and are major antigens in smallpox vaccine. These antibodies are useful for tracking distinct steps in virion assembly and for studying the B cell epitopes in smallpox vaccine.

Original languageEnglish (US)
Title of host publicationVaccinia Virus and Poxvirology
Subtitle of host publicationMethods and Protocols
PublisherHumana Press
Pages219-232
Number of pages14
ISBN (Print)9781617798757
DOIs
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
Volume890
ISSN (Print)1064-3745

Keywords

  • Hybridoma
  • Monoclonal antibody
  • Poxvirus
  • Vaccinia virus

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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