TY - JOUR
T1 - Generation and characterization of a large panel of murine monoclonal antibodies against vaccinia virus
AU - Meng, Xiangzhi
AU - Zhong, Youmin
AU - Embry, Addie
AU - Yan, Bo
AU - Lu, Shan
AU - Zhong, Guangming
AU - Xiang, Yan
N1 - Funding Information:
We thank Dr. Bernard Moss for A33-deletion virus and Dr. Michael Merchlinsky for WR148-deletion virus. We thank Cao Jie for technical assistance. This work was supported by NIH grants AI079217 (Y.X.) and AI064537 (GMZ) .
PY - 2011/1/20
Y1 - 2011/1/20
N2 - Vaccinia virus (VACV), the vaccine for smallpox, induces an antibody response that is largely responsible for conferring protection. Here, we studied the antibody response to VACV by generating and characterizing B cell hybridomas from a mouse immunized with VACV. Antibodies from 66 hybridomas were found to recognize 11 VACV antigens (D8, A14, WR148, D13, H3, A56, A33, C3, B5, A10 and F13), 10 of which were previously recognized as major antigens in smallpox vaccine by a microarray of VACV proteins produced with a prokaryotic expression system. VACV C3 protein, which was not detected as a target of antibody response by the proteome array, was recognized by two hybridomas, suggesting that selection of hybridomas based on immune recognition of infected cells has the advantage of detecting additional antibody response to native VACV antigens. In addition, these monoclonal antibodies are valuable reagents for studying poxvirus biology and protective mechanism of smallpox vaccine.
AB - Vaccinia virus (VACV), the vaccine for smallpox, induces an antibody response that is largely responsible for conferring protection. Here, we studied the antibody response to VACV by generating and characterizing B cell hybridomas from a mouse immunized with VACV. Antibodies from 66 hybridomas were found to recognize 11 VACV antigens (D8, A14, WR148, D13, H3, A56, A33, C3, B5, A10 and F13), 10 of which were previously recognized as major antigens in smallpox vaccine by a microarray of VACV proteins produced with a prokaryotic expression system. VACV C3 protein, which was not detected as a target of antibody response by the proteome array, was recognized by two hybridomas, suggesting that selection of hybridomas based on immune recognition of infected cells has the advantage of detecting additional antibody response to native VACV antigens. In addition, these monoclonal antibodies are valuable reagents for studying poxvirus biology and protective mechanism of smallpox vaccine.
KW - Antibody
KW - Poxvirus
KW - Smallpox
KW - Vaccinia
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U2 - 10.1016/j.virol.2010.10.019
DO - 10.1016/j.virol.2010.10.019
M3 - Article
C2 - 21056889
AN - SCOPUS:78650260578
SN - 0042-6822
VL - 409
SP - 271
EP - 279
JO - Virology
JF - Virology
IS - 2
ER -