@inbook{b44b39f83f2746cf868e6ed2df05a128,
title = "Generating neural stem cells from iPSCs with dopaminergic neurons reporter gene",
abstract = "Genetic reporters offer attractive approaches to investigate gene expression, gene function, and spatiotemporal assessment in vitro and in vivo. Tyrosine hydroxylase (TH) is the rate-limiting enzyme for the biosynthesis of the dopamine neurotransmitter, and thus it has been used as a reliable marker for dopaminergic neurons in vitro and in vivo. Herein we describe a method for making iPSC lines with TH-green fluorescent protein reporter gene using CRISPR/Cas9 technique.",
keywords = "CRISPR/Cas9, Human pluripotent stem cells, Neural stem cell, Parkinson{\textquoteright}s disease, Reporter, Tyrosine hydroxylase",
author = "Hyenjong Hong and Daadi, {Marcel M.}",
note = "Publisher Copyright: {\textcopyright} 2019, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2019",
doi = "10.1007/978-1-4939-9007-8_9",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "119--128",
booktitle = "Methods in Molecular Biology",
address = "United States",
}