Generating neural stem cells from iPSCs with dopaminergic neurons reporter gene

Hyenjong Hong, Marcel M. Daadi

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Scopus citations


Genetic reporters offer attractive approaches to investigate gene expression, gene function, and spatiotemporal assessment in vitro and in vivo. Tyrosine hydroxylase (TH) is the rate-limiting enzyme for the biosynthesis of the dopamine neurotransmitter, and thus it has been used as a reliable marker for dopaminergic neurons in vitro and in vivo. Herein we describe a method for making iPSC lines with TH-green fluorescent protein reporter gene using CRISPR/Cas9 technique.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Number of pages10
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • CRISPR/Cas9
  • Human pluripotent stem cells
  • Neural stem cell
  • Parkinson’s disease
  • Reporter
  • Tyrosine hydroxylase

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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