General Regulatory Factors (GRFs) as genome partitioners

Geneviève Fourel, Tsuyoshi Miyake, Pierre Antoine Defossez, Rong Li, Éric Gilson

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Insulators are sequences that uncouple adjacent chromosome domains. Here we have shown that Saccharomyces cerevisiae Rap1p and Abf1p proteins are endowed with a potent insulating capacity. Insulating domains in Rap1p coincide with previously described transcription activation domains, whereas four adjacent subdomains spanning the whole of the Abf1p C terminus (440-731) were found to display autonomous insulating capacity. That both Rap1p and Abf1p silencing domains either contain or largely overlap with an insulating domain suggests that insulation conveys some undefined chromosome organization capacity that also contributes a function in silencing. Together with Reb1p and Tbf1p, previously involved in the activity of Saccharomyces cerevisiae subtelomeric insulators, insulating potential emerges as a supplementary common property of General Regulatory Factors (GRFs). Thus GRFs, which bind to sites scattered throughout the genome within promoters, would not only play a key role in regulating gene expression but also partition the genome in functionally independent domains.

Original languageEnglish (US)
Pages (from-to)41736-41743
Number of pages8
JournalJournal of Biological Chemistry
Volume277
Issue number44
DOIs
Publication statusPublished - Nov 1 2002

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Fourel, G., Miyake, T., Defossez, P. A., Li, R., & Gilson, É. (2002). General Regulatory Factors (GRFs) as genome partitioners. Journal of Biological Chemistry, 277(44), 41736-41743. https://doi.org/10.1074/jbc.M202578200