Gene Transfer and Expression Studies in Cultured Avian Neural Crest Cells Differentiating Into Melanocytes

JUERGEN R. VIELKIND; KRISTTNE S. VOGEL

doi:10.1111/j.1600-0749.1989.tb00157.x

Gene Transfer and Expression Studies in Cultured Avian Neural Crest Cells Differentiating Into Melanocytes

JUERGEN R. VIELKIND, KRISTTNE S. VOGEL

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Neural crest cells obtained from explanted neural tubes take up, express, and retain exogenous DNA applied by the CaPO₄ co‐precipitation method during their differentiation into melanocytes. High efficiencies of gene transfer were obtained with both supercoiled DNA and intact phage particles; linear DNA or DNA from the phage yielded very low efficiencies. There is some evidence that transferred gene expression is differentiation dependent. The system should be useful for studies concerned with the analysis of cell developmental genes and their regulatory elements.

Original language	English (US)
Pages (from-to)	44-52
Number of pages	9
Journal	Pigment Cell Research
Volume	2
Issue number	1
DOIs	https://doi.org/10.1111/j.1600-0749.1989.tb00157.x
State	Published - Jan 1989
Externally published	Yes

Keywords

Melanogenesis
Neural tube explants
Recombinant phage
Transfection

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science
Developmental Biology
Clinical Biochemistry
Cell Biology

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N2 - Neural crest cells obtained from explanted neural tubes take up, express, and retain exogenous DNA applied by the CaPO4 co‐precipitation method during their differentiation into melanocytes. High efficiencies of gene transfer were obtained with both supercoiled DNA and intact phage particles; linear DNA or DNA from the phage yielded very low efficiencies. There is some evidence that transferred gene expression is differentiation dependent. The system should be useful for studies concerned with the analysis of cell developmental genes and their regulatory elements.

AB - Neural crest cells obtained from explanted neural tubes take up, express, and retain exogenous DNA applied by the CaPO4 co‐precipitation method during their differentiation into melanocytes. High efficiencies of gene transfer were obtained with both supercoiled DNA and intact phage particles; linear DNA or DNA from the phage yielded very low efficiencies. There is some evidence that transferred gene expression is differentiation dependent. The system should be useful for studies concerned with the analysis of cell developmental genes and their regulatory elements.

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KW - Recombinant phage

KW - Transfection

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Gene Transfer and Expression Studies in Cultured Avian Neural Crest Cells Differentiating Into Melanocytes

Abstract

Keywords

ASJC Scopus subject areas

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