Gene Transfer and Expression Studies in Cultured Avian Neural Crest Cells Differentiating Into Melanocytes

JUERGEN R. VIELKIND, KRISTTNE S. VOGEL

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Neural crest cells obtained from explanted neural tubes take up, express, and retain exogenous DNA applied by the CaPO4 co‐precipitation method during their differentiation into melanocytes. High efficiencies of gene transfer were obtained with both supercoiled DNA and intact phage particles; linear DNA or DNA from the phage yielded very low efficiencies. There is some evidence that transferred gene expression is differentiation dependent. The system should be useful for studies concerned with the analysis of cell developmental genes and their regulatory elements.

Original languageEnglish (US)
Pages (from-to)44-52
Number of pages9
JournalPigment Cell Research
Volume2
Issue number1
DOIs
StatePublished - Jan 1989
Externally publishedYes

    Fingerprint

Keywords

  • Melanogenesis
  • Neural tube explants
  • Recombinant phage
  • Transfection

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science
  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this