Gene sequence and primary structure of mitochondrial malate dehydrogenase from Saccharomyces cerevisiae

Leslie M. Thompson, Pamela Sutherland, Joan S. Steffan, Lee McAlister-Henn

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The nucleotide sequence was determined for a 1.5-kilobase genomic fragment containing the mitochondrial malate dehydrogenase gene (MDH1) of Saccharomyces cerevisiae. The open-reading frame encodes a precursor form of the mature enzyme containing an amino-terminal extension of 17 amino acid residues. In vitro translation experiments confirm that the initial translation product of MDH1 is larger than the mature polypeptide. Transcription of MDH1 initiates at several sites from 83 to 97 nucleotides 5′ of the translational start site. Alignment of the amino acid sequence for the mature yeast enzyme with those for mammalian mitochondrial and for Escherichia coli malate dehydrogenases reveals polypeptides of very similar sizes with identical amino acids at 54% and 48% of the residue positions, respectively. The amino acid sequences of the yeast and mammalian mitochondrial targeting sequences are similar but less related than the mature polypeptides. The yeast MDH1 gene is shown to reside on chromosome XI.

Original languageEnglish (US)
Pages (from-to)8393-8400
Number of pages8
JournalBiochemistry
Volume27
Issue number22
StatePublished - 1988
Externally publishedYes

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Malate Dehydrogenase
Yeast
Saccharomyces cerevisiae
Genes
Yeasts
Amino Acids
Peptides
Amino Acid Sequence
Nucleotides
Enzymes
Open Reading Frames
Transcription
Chromosomes
Escherichia coli
Experiments

ASJC Scopus subject areas

  • Biochemistry

Cite this

Thompson, L. M., Sutherland, P., Steffan, J. S., & McAlister-Henn, L. (1988). Gene sequence and primary structure of mitochondrial malate dehydrogenase from Saccharomyces cerevisiae. Biochemistry, 27(22), 8393-8400.

Gene sequence and primary structure of mitochondrial malate dehydrogenase from Saccharomyces cerevisiae. / Thompson, Leslie M.; Sutherland, Pamela; Steffan, Joan S.; McAlister-Henn, Lee.

In: Biochemistry, Vol. 27, No. 22, 1988, p. 8393-8400.

Research output: Contribution to journalArticle

Thompson, LM, Sutherland, P, Steffan, JS & McAlister-Henn, L 1988, 'Gene sequence and primary structure of mitochondrial malate dehydrogenase from Saccharomyces cerevisiae', Biochemistry, vol. 27, no. 22, pp. 8393-8400.
Thompson LM, Sutherland P, Steffan JS, McAlister-Henn L. Gene sequence and primary structure of mitochondrial malate dehydrogenase from Saccharomyces cerevisiae. Biochemistry. 1988;27(22):8393-8400.
Thompson, Leslie M. ; Sutherland, Pamela ; Steffan, Joan S. ; McAlister-Henn, Lee. / Gene sequence and primary structure of mitochondrial malate dehydrogenase from Saccharomyces cerevisiae. In: Biochemistry. 1988 ; Vol. 27, No. 22. pp. 8393-8400.
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