Gene profiling approach in the analysis of lymphotoxin and TNF deficiencies

A. N. Shakhov, I. G. Lyakhov, A. V. Tumanov, A. V. Rubtsov, L. N. Drutskaya, M. W. Marino, S. A. Nedospasov

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


Mice with combined lymphotoxin-α (LTα) and tumor necrosis factor (TNF) deficiencies show defects in the structure of peripheral lymphoid organs such as spleen, lymph nodes, and gut-associated lymphoid tissues. To identify genes associated with this defective phenotype in spleen, we applied a gene profiling approach, including subtractive cloning and gene array hybridizations, to mice with combined TNF/LT deficiency. The differentially expressed genes identified by these techniques was then evaluated by Northern blot analysis for splenic expression in knockout mice with single LTα or single TNF deficiency. Most of the genes detected in this analysis are directly or indirectly associated with disrupted LT and not TNF signaling.

Original languageEnglish (US)
Pages (from-to)151-157
Number of pages7
JournalJournal of Leukocyte Biology
Issue number1
StatePublished - 2000
Externally publishedYes


  • Gene arrays
  • Knockout mice
  • Spleen
  • Subtractive cloning

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology


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