Gene expression after treatment with hydrogen peroxide, menadione, or t-butyl hydroperoxide in breast cancer cells

Yao Yu Eric Chuang, Yidong Chen, Gadisetti V.R. Chandramouli, John A. Cook, Deborah Coffin, Mong Hsun Tsai, William DeGraff, Hailing Yan, Shuping Zhao, Angelo Russo, Edison T. Liu, James B. Mitchell

Research output: Contribution to journalArticlepeer-review

106 Scopus citations

Abstract

Global gene expression patterns in breast cancer cells after treatment with oxidants (hydrogen peroxide, menadione, and t-butyl hydroperoxide) were investigated in three replicate experiments. RNA collected after treatment (at 1, 3, 7, and 24 h) rather than after a single time point, enabled an analysis of gene expression patterns. Using a 17,000 microarray, template-based clustering and multidimensional scaling analysis of the gene expression over the entire time course identified 421 genes as being either up- or down-regulated by the three oxidants. In contrast, only 127 genes were identified for any single time point and a 2-fold change criteria. Surprisingly, the patterns of gene induction were highly similar among the three oxidants; however, differences were observed, particularly with respect to p53, IL-6, and heat-shock related genes. Replicate experiments increased the statistical confidence of the study, whereas changes in gene expression patterns over a time course demonstrated significant additional information versus a single time point. Analyzing the three oxidants simultaneously by template cluster analysis identified genes that heretofore have not been associated with oxidative stress.

Original languageEnglish (US)
Pages (from-to)6246-6254
Number of pages9
JournalCancer Research
Volume62
Issue number21
StatePublished - Nov 1 2002
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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