Gender differences and time course of castration-induced changes in porphyrins, indoles, and proteins in the Harderian glands of the Syrian hamster

G. R. Buzzell, A. Menendez-Pelaez, V. Chlumecky, R. J. Reiter

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Sexual differences and the effects of orchidectomy were determined for porphyrin and melatonin concentrations and for the activities of the enzymes N-acetyltransferase and hydroxyindole-O-methyltransferase, which synthesize melatonin from serotonin, in the Harderian glands of the Syrian hamster. Porphyrin concentrations in intact males were about 1/400th those of intact females. Castration for 1 week increased male Harderian porphyrin concentrations 10-fold; by 3 weeks, castrated male porphyrin levels were 140 times those of control values. N-Acetyltransferase activity in intact male Harderian glands was about 4 times that of females. Castration led to a drop in N-acetyltransferase activity to female levels within 2 weeks. Hydroxyindole-O-methyltransferase activity was 7 times higher in females than in males and castration had no effect on male Harderian hydroxyindole-O-methyltransferase activity. Neither gender nor castration influenced Harderian melatonin concentrations. Soluble proteins in Harderian glands from male and female hamsters and from male hamsters castrated for 1 and 4 weeks were examined by sodium docecyl sulfate - polyacrylamide gel electrophoresis. The gel profiles revealed several differences among the protein distribution in male and female gland lysates. Orchidectomy led to a female protein pattern within 4 weeks.

Original languageEnglish (US)
Pages (from-to)1814-1818
Number of pages5
JournalCanadian Journal of Physiology and Pharmacology
Volume69
Issue number12
DOIs
StatePublished - Jan 1 1991

Keywords

  • Harderian glands
  • N-acetyltransfe rase
  • SDS-PAGE
  • hamsters
  • hydroxyindole-O-methyltransferase
  • melatonin
  • porphyrins
  • proteins
  • sexual dimorphism

ASJC Scopus subject areas

  • Physiology
  • Pharmacology
  • Physiology (medical)

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