TY - JOUR
T1 - Gender-associated differences in the development of 5-aminolevulinate synthase gene expression in the Harderian gland of Syrian hamsters
AU - Rodriguez, Carmen
AU - Menendez-Pelaez, Armando
AU - Vaughan, Mary K.
AU - Reiter, Russel J.
N1 - Funding Information:
Research supported by NSF grant IBN 91-21262 (RJ.R.) and DGICYT PM894086 (A.M.-P.). C.R. ac-knowledgesa University of Oviedo fellowship.A .M.-P. was the recipient of a Fogarty fellowship.T he cDNA for ALV-S was kindly provided by Dr. B.K. May (Adelaide, Australia).
PY - 1993/6
Y1 - 1993/6
N2 - The mRNA levels for aminolevulinate synthase (ALV-S), the rate-limiting enzyme in porphyrin synthesis, were studied in male and female Syrian hamsters during postnatal development. Sex-associated differences in the expression of ALV-S gene were evident at the end of the third week of postnatal development. Serum levels of luteinizing hormone (LH), testosterone, cortisol, thyroid hormones and insulin-like growth factor were also studied in order to correlate their concentrations with the mRNA levels for ALV-S. Among these hormones, serum LH levels showed a positive correlation with the ALV-S mRNA levels. However, the expected negative correlation with testosterone levels was not clearly observed. Thus, in order to test the effects of testosterone on ALV-S gene expression, 11-day-old male and female Syrian hamsters and adult female hamsters were injected with 50 μg of testosterone for 4 days. Testosterone administration decreased the levels of ALV-S mRNA in the adult females but did not influence those of young females. The possible explanation for the insensitivity to testosterone during these postnatal stages might involve the maturational state of androgen receptors in the Harderian glands.
AB - The mRNA levels for aminolevulinate synthase (ALV-S), the rate-limiting enzyme in porphyrin synthesis, were studied in male and female Syrian hamsters during postnatal development. Sex-associated differences in the expression of ALV-S gene were evident at the end of the third week of postnatal development. Serum levels of luteinizing hormone (LH), testosterone, cortisol, thyroid hormones and insulin-like growth factor were also studied in order to correlate their concentrations with the mRNA levels for ALV-S. Among these hormones, serum LH levels showed a positive correlation with the ALV-S mRNA levels. However, the expected negative correlation with testosterone levels was not clearly observed. Thus, in order to test the effects of testosterone on ALV-S gene expression, 11-day-old male and female Syrian hamsters and adult female hamsters were injected with 50 μg of testosterone for 4 days. Testosterone administration decreased the levels of ALV-S mRNA in the adult females but did not influence those of young females. The possible explanation for the insensitivity to testosterone during these postnatal stages might involve the maturational state of androgen receptors in the Harderian glands.
KW - 5-Aminolevulinate synthase
KW - Development testosterone
KW - Hamster
KW - Harderian glands
KW - Insulin-like growth factor
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U2 - 10.1016/0303-7207(93)90120-9
DO - 10.1016/0303-7207(93)90120-9
M3 - Article
C2 - 8349026
AN - SCOPUS:0027309265
VL - 93
SP - 167
EP - 173
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
SN - 0303-7207
IS - 2
ER -