Gel electrophoresis of micron-sized particles: A problem and a solution

G. A. Griess, Philip Serwer

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The gel electrophoresis of spherical particles with a radius above 0.2 μm has not been reported yet. In the present study, video phase-contrast light microscopy is used to observe the motion of individual latex spheres, 0.52 μm in radius, during electrophoresis in 0.1% agarose gels. At 2 V/cm, the spheres initially migrate in the direction of the electrical field. However, each sphere eventually undergoes a cessation of all motion. Brownian motion is restored when the electrical potential gradient is reduced to zero. Arrest can be prevented by periodically inverating the direction of the electrical field. These observations are explained by electrical field-induced steric trapping of the spheres by gel fibers. Inversion of the electrical field should assist the application of agarose gel electrophoresis to micron-sized cellular organelles and cells.

Original languageEnglish (US)
Pages (from-to)1863-1866
Number of pages4
JournalBiopolymers
Volume29
Issue number14
DOIs
StatePublished - 1990

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Electrophoresis
Particles (particulate matter)
Gels
Sepharose
Phase-Contrast Microscopy
Video Microscopy
Agar Gel Electrophoresis
Microspheres
Organelles
Brownian movement
Latex
Latexes
Optical microscopy
Light
Fibers
Direction compound

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

Gel electrophoresis of micron-sized particles : A problem and a solution. / Griess, G. A.; Serwer, Philip.

In: Biopolymers, Vol. 29, No. 14, 1990, p. 1863-1866.

Research output: Contribution to journalArticle

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