Gastrointestinal pythiosis with concurrent presumptive gastrointestinal basidiobolomycosis in a Boxer dog

Joseph C. Parambeth, Sara D. Lawhon, Joanne Mansell, Jing Wu, Sabrina D. Clark, Deanna Sutton, Connie Gibas, Nathan Wiederhold, Alexandra N. Myers, Mark C. Johnson, Gwendolyn J. Levine, Samantha Schlemmer, Catherine Ruoff, Anitha Isaiah, Kate E. Creevy

Research output: Contribution to journalArticle

Abstract

A 2-year-old female spayed Boxer dog was presented for a 1-month history of progressive hemorrhagic diarrhea with tenesmus and weight loss despite trial courses of antibiotics and diet change. Abdominal ultrasound revealed severe, focal thickening, and loss of normal architecture of the colonic wall with abdominal lymphadenomegaly. Dry-mount fecal cytology, performed on several consecutive days, consistently revealed numerous, round, 16-20 μm structures with basophilic, granular content, and a thin cell wall. Transmission electron microscopy identified these structures as fungi. Culture, polymerase chain reaction (PCR), and sequencing of the internal transcribed spacer, D1/D2 regions, and DNA-directed RNA polymerase II core subunit (RPB2) confirmed the presence of Basidiobolus microsporus in the feces. Biopsies collected via ileocolonoscopy revealed marked, multifocal, chronic, neutrophilic, and eosinophilic ileitis and colitis with ulceration, granulation tissue, and intralesional hyphae (identified with Gomori methenamine silver stain). A Pythium enzyme-linked immunosorbent assay and Pythium-specific PCR performed on the formalin-fixed paraffin-embedded biopsy specimens were positive while Basidiobolus-specific PCR was negative, thus confirming a diagnosis of pythiosis. This report describes a fatal case of colonic and intestinal pythiosis with the presence of fecal Basidiobolus sp. spores, suggestive of concurrent gastrointestinal basidiobolomycosis.

Original languageEnglish (US)
Pages (from-to)83-88
Number of pages6
JournalVeterinary Clinical Pathology
Volume48
Issue number1
DOIs
StatePublished - Mar 1 2019

Fingerprint

Pythiosis
Entomophthorales
Basidiobolus
Boxer (dog breed)
Pythium
polymerase chain reaction
Dogs
DNA-directed RNA polymerase
Polymerase Chain Reaction
dogs
biopsy
Ileitis
Methenamine
Biopsy
granulation tissue
Granulation Tissue
Hyphae
RNA Polymerase II
colitis
Abdominal Wall

Keywords

  • canine
  • diarrhea
  • oomycosis
  • zygomycosis

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Parambeth, J. C., Lawhon, S. D., Mansell, J., Wu, J., Clark, S. D., Sutton, D., ... Creevy, K. E. (2019). Gastrointestinal pythiosis with concurrent presumptive gastrointestinal basidiobolomycosis in a Boxer dog. Veterinary Clinical Pathology, 48(1), 83-88. https://doi.org/10.1111/vcp.12720

Gastrointestinal pythiosis with concurrent presumptive gastrointestinal basidiobolomycosis in a Boxer dog. / Parambeth, Joseph C.; Lawhon, Sara D.; Mansell, Joanne; Wu, Jing; Clark, Sabrina D.; Sutton, Deanna; Gibas, Connie; Wiederhold, Nathan; Myers, Alexandra N.; Johnson, Mark C.; Levine, Gwendolyn J.; Schlemmer, Samantha; Ruoff, Catherine; Isaiah, Anitha; Creevy, Kate E.

In: Veterinary Clinical Pathology, Vol. 48, No. 1, 01.03.2019, p. 83-88.

Research output: Contribution to journalArticle

Parambeth, JC, Lawhon, SD, Mansell, J, Wu, J, Clark, SD, Sutton, D, Gibas, C, Wiederhold, N, Myers, AN, Johnson, MC, Levine, GJ, Schlemmer, S, Ruoff, C, Isaiah, A & Creevy, KE 2019, 'Gastrointestinal pythiosis with concurrent presumptive gastrointestinal basidiobolomycosis in a Boxer dog', Veterinary Clinical Pathology, vol. 48, no. 1, pp. 83-88. https://doi.org/10.1111/vcp.12720
Parambeth, Joseph C. ; Lawhon, Sara D. ; Mansell, Joanne ; Wu, Jing ; Clark, Sabrina D. ; Sutton, Deanna ; Gibas, Connie ; Wiederhold, Nathan ; Myers, Alexandra N. ; Johnson, Mark C. ; Levine, Gwendolyn J. ; Schlemmer, Samantha ; Ruoff, Catherine ; Isaiah, Anitha ; Creevy, Kate E. / Gastrointestinal pythiosis with concurrent presumptive gastrointestinal basidiobolomycosis in a Boxer dog. In: Veterinary Clinical Pathology. 2019 ; Vol. 48, No. 1. pp. 83-88.
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