Gamma delta t cells regulate wound myeloid CELL activity after burn

Meenakshi Rani, Qiong Zhang, Martin G Schwacha

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Major burns induce immune complications, which are associated with myeloid cell activation by ill-defined mechanisms. Although γδ T cells have been shown to be important in postinjury inflammation and wound healing, their role in the regulation of myeloid cells remains unknown. To study this, wild-type (WT) and γδ T cell-deficient (δTCR) mice were subjected to major burn (25% total body surface area, third degree) or sham treatment. At 3 days thereafter, skin samples were assayed for cytokine content or used to isolate single cells that were used for myeloid cell characterization by flow cytometry. The number of CD11b myeloid cells increased by approximately 75% in the wound skin of WT mice. This influx was caused by increased myeloid-derived suppressor cells (CD11b GR1) whose numbers increased 19-fold compared with those of sham skin. In contrast, macrophage (MØ; CD11b F4/80) numbers decreased by approximately 50% after burn. In δTCR mice, burn increased the myeloid cell numbers approximately 5-fold. The increase in myeloid cells at the injury site of δTCR mice was caused by both a myeloid-derived suppressor cell (50-fold) and a MØ (2-fold) influx. Burn increased skin cytokine levels for a number of prototypic inflammatory cytokines (interleukin 1β, interleukin 6, tumor necrosis factor-α, macrophage inflammatory protein [MIP] 1β, etc). Tumor necrosis factor-α, MIP-1α, and MIP-1β levels were further elevated (2- to 3-fold) in the injured skin of δTCR mice compared with those of WT mice. In conclusion, these data show that γδ T cells regulate myeloid cell infiltration of the wound site and act to quell inflammation, thereby promoting the transition to the proliferative phase of wound healing.

Original languageEnglish (US)
Pages (from-to)133-141
Number of pages9
JournalShock
Volume42
Issue number2
DOIs
StatePublished - 2014

Fingerprint

Somatostatin-Secreting Cells
Myeloid Cells
Macrophage Inflammatory Proteins
Wounds and Injuries
Skin
Cytokines
T-Lymphocytes
Wound Healing
Tumor Necrosis Factor-alpha
Inflammation
Body Surface Area
Interleukin-1
Burns
Interleukin-6
Flow Cytometry
Cell Count
Macrophages
Placebos

Keywords

  • cytokines
  • inflammation
  • Injury
  • macrophage
  • MDSC

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Emergency Medicine
  • Medicine(all)

Cite this

Gamma delta t cells regulate wound myeloid CELL activity after burn. / Rani, Meenakshi; Zhang, Qiong; Schwacha, Martin G.

In: Shock, Vol. 42, No. 2, 2014, p. 133-141.

Research output: Contribution to journalArticle

Rani, Meenakshi ; Zhang, Qiong ; Schwacha, Martin G. / Gamma delta t cells regulate wound myeloid CELL activity after burn. In: Shock. 2014 ; Vol. 42, No. 2. pp. 133-141.
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abstract = "Major burns induce immune complications, which are associated with myeloid cell activation by ill-defined mechanisms. Although γδ T cells have been shown to be important in postinjury inflammation and wound healing, their role in the regulation of myeloid cells remains unknown. To study this, wild-type (WT) and γδ T cell-deficient (δTCR) mice were subjected to major burn (25{\%} total body surface area, third degree) or sham treatment. At 3 days thereafter, skin samples were assayed for cytokine content or used to isolate single cells that were used for myeloid cell characterization by flow cytometry. The number of CD11b myeloid cells increased by approximately 75{\%} in the wound skin of WT mice. This influx was caused by increased myeloid-derived suppressor cells (CD11b GR1) whose numbers increased 19-fold compared with those of sham skin. In contrast, macrophage (M{\O}; CD11b F4/80) numbers decreased by approximately 50{\%} after burn. In δTCR mice, burn increased the myeloid cell numbers approximately 5-fold. The increase in myeloid cells at the injury site of δTCR mice was caused by both a myeloid-derived suppressor cell (50-fold) and a M{\O} (2-fold) influx. Burn increased skin cytokine levels for a number of prototypic inflammatory cytokines (interleukin 1β, interleukin 6, tumor necrosis factor-α, macrophage inflammatory protein [MIP] 1β, etc). Tumor necrosis factor-α, MIP-1α, and MIP-1β levels were further elevated (2- to 3-fold) in the injured skin of δTCR mice compared with those of WT mice. In conclusion, these data show that γδ T cells regulate myeloid cell infiltration of the wound site and act to quell inflammation, thereby promoting the transition to the proliferative phase of wound healing.",
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