Gamete–specific methylation correlates with imprinting of the murine Xist gene

Mira Ariel, Edward Robinson, John R. McCarrey, Howard Cedar

Research output: Contribution to journalArticlepeer-review

99 Scopus citations


We have investigated the potential role of DNA methylation as a regulator of imprinted Xist expression in mouse preimplantation embryos. The active paternal allele was found to be unmodified in sperm at CpG loci near the 5′ end of the gene transcription unit. In contrast, on the inactive maternal allele, these same sites are initially methylated in the oocyte and then remain modified in the early embryo. In the male germ line, these methyl moieties are removed during spermatogenesis, and this occurs before the programmed reactivation of Xist in the testis. This represents a clear–cut example of a potential methylation imprinting signal that is reprogrammable and gamete derived.

Original languageEnglish (US)
Pages (from-to)312-315
Number of pages4
JournalNature Genetics
Issue number3
StatePublished - Mar 1995
Externally publishedYes

ASJC Scopus subject areas

  • Genetics


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