Galanin-mediated anxiolytic effect in rat central amygdala is not a result of corelease from noradrenergic terminals

Gabe Barrera, Angelica Hernandez, Jean Francois Poulin, Sylvie Laforest, Guy Drolet, David A. Morilak

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Galanin is colocalized extensively with norepinephrine in brain. Although this suggests possible activity-dependent neurotransmitter interactions, the functional significance of such colocalization remains elusive. Previously, we showed that enhancing stress-activation of the noradrenergic system by yohimbine pretreatment released galanin in central amygdala, attenuating the anxiety-like behavioral response to stress on the elevated plus-maze. The present study was conducted to determine, in this context, whether galanin was indeed coreleased from noradrenergic terminals, or instead from another galanin afferent or local stress-responsive galanin neurons in the amygdala. In experiment 1, galanin-mediated anxiolytic effects on the plus-maze following yohimbine + stress were unaltered by lesioning the noradrenergic innervation of central amygdala. In experiment 2, combining immunohistochemistry and in situ hybridization, galanin neurons specifically activated by yohimbine + stress treatment were found only in the locus coeruleus and intraamygdalar bed nucleus of the stria terminalis, adjacent to central amygdala. In experiment 3, retrograde tracing combined with in situ hybridization revealed few if any galanin cells projecting to central amygdala in locus coeruleus or nucleus tractus solitarius, sources of noradrenergic innervation. Indeed, few retrogradely-labeled galanin neurons were observed anywhere in the brain, including a small number in the intraamygdalar bed nucleus. Together, these results suggest that stress following yohimbine may have induced galanin release from an afferent to central amygdala originating in the bed nucleus, or from local neurons in the intraamygdalar bed nucleus, but that anxiolytic effects exerted by galanin in this context of elevated noradrenergic activity were not the result of corelease from noradrenergic terminals innervating central amygdala.

Original languageEnglish (US)
Pages (from-to)27-40
Number of pages14
JournalSynapse
Volume59
Issue number1
DOIs
StatePublished - Jan 1 2006

Keywords

  • Anxiety
  • Elevated plus-maze
  • Neuropeptides
  • Norepinephrine
  • Stress
  • Yohimbine

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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