Abstract
This report demonstrates that galactosyl ceramide (GalCer) or a molecule derived from it may serve as an alternative receptor for human immunodeficiency virus in the nervous system. Recombinant gp120, an envelope glycoprotein of human immunodeficiency virus type 1, specifically binds to GalCer and its derivatives. This specificity was studied by inhibiting binding of radioiodinated gp120 to GalCer with antibodies to GalCer, antibodies to gp120, and an excess of unlabeled gp120. Binding activity was also removed by absorbing gp120 with liposomes containing GalCer. In addition, studies using natural and semisynthetic lipids indicate that the linkage between galactose and ceramide is essential for binding. The significance of an alternative receptor for human immunodeficiency virus in the nervous system is discussed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 7131-7134 |
| Number of pages | 4 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 88 |
| Issue number | 16 |
| State | Published - 1991 |
| Externally published | Yes |
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Keywords
- AIDS
- Demyelination
- Glycolipid
- Sulfatide
ASJC Scopus subject areas
- General
- Genetics
Cite this
Galactosyl ceramide or a derivative is an essential component of the neural receptor for human immunodeficiency virus type 1 envelope glycoprotein gp120. / Bhat, Shama; Spitalnik, Steven L.; Gonzalez-Scarano, Francisco; Silberberg, Donald H.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, No. 16, 1991, p. 7131-7134.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Galactosyl ceramide or a derivative is an essential component of the neural receptor for human immunodeficiency virus type 1 envelope glycoprotein gp120
AU - Bhat, Shama
AU - Spitalnik, Steven L.
AU - Gonzalez-Scarano, Francisco
AU - Silberberg, Donald H.
PY - 1991
Y1 - 1991
N2 - This report demonstrates that galactosyl ceramide (GalCer) or a molecule derived from it may serve as an alternative receptor for human immunodeficiency virus in the nervous system. Recombinant gp120, an envelope glycoprotein of human immunodeficiency virus type 1, specifically binds to GalCer and its derivatives. This specificity was studied by inhibiting binding of radioiodinated gp120 to GalCer with antibodies to GalCer, antibodies to gp120, and an excess of unlabeled gp120. Binding activity was also removed by absorbing gp120 with liposomes containing GalCer. In addition, studies using natural and semisynthetic lipids indicate that the linkage between galactose and ceramide is essential for binding. The significance of an alternative receptor for human immunodeficiency virus in the nervous system is discussed.
AB - This report demonstrates that galactosyl ceramide (GalCer) or a molecule derived from it may serve as an alternative receptor for human immunodeficiency virus in the nervous system. Recombinant gp120, an envelope glycoprotein of human immunodeficiency virus type 1, specifically binds to GalCer and its derivatives. This specificity was studied by inhibiting binding of radioiodinated gp120 to GalCer with antibodies to GalCer, antibodies to gp120, and an excess of unlabeled gp120. Binding activity was also removed by absorbing gp120 with liposomes containing GalCer. In addition, studies using natural and semisynthetic lipids indicate that the linkage between galactose and ceramide is essential for binding. The significance of an alternative receptor for human immunodeficiency virus in the nervous system is discussed.
KW - AIDS
KW - Demyelination
KW - Glycolipid
KW - Sulfatide
UR - http://www.scopus.com/inward/record.url?scp=0025738161&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025738161&partnerID=8YFLogxK
M3 - Article
C2 - 1871126
AN - SCOPUS:0025738161
VL - 88
SP - 7131
EP - 7134
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 16
ER -