Galactosyl ceramide or a derivative is an essential component of the neural receptor for human immunodeficiency virus type 1 envelope glycoprotein gp120

Shama Bhat; Steven L. Spitalnik; Francisco Gonzalez-Scarano; Donald H. Silberberg

doi:10.1073/pnas.88.16.7131

Galactosyl ceramide or a derivative is an essential component of the neural receptor for human immunodeficiency virus type 1 envelope glycoprotein gp120

Shama Bhat, Steven L. Spitalnik, Francisco Gonzalez-Scarano, Donald H. Silberberg

Neurology

Research output: Contribution to journal › Article › peer-review

215 Scopus citations

Abstract

This report demonstrates that galactosyl ceramide (GalCer) or a molecule derived from it may serve as an alternative receptor for human immunodeficiency virus in the nervous system. Recombinant gp120, an envelope glycoprotein of human immunodeficiency virus type 1, specifically binds to GalCer and its derivatives. This specificity was studied by inhibiting binding of radioiodinated gp120 to GalCer with antibodies to GalCer, antibodies to gp120, and an excess of unlabeled gp120. Binding activity was also removed by absorbing gp120 with liposomes containing GalCer. In addition, studies using natural and semisynthetic lipids indicate that the linkage between galactose and ceramide is essential for binding. The significance of an alternative receptor for human immunodeficiency virus in the nervous system is discussed.

Original language	English (US)
Pages (from-to)	7131-7134
Number of pages	4
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	88
Issue number	16
DOIs	https://doi.org/10.1073/pnas.88.16.7131
State	Published - 1991

Keywords

AIDS
Demyelination
Glycolipid
Sulfatide

ASJC Scopus subject areas

General

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10.1073/pnas.88.16.7131

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Bhat, S., Spitalnik, S. L., Gonzalez-Scarano, F., & Silberberg, D. H. (1991). Galactosyl ceramide or a derivative is an essential component of the neural receptor for human immunodeficiency virus type 1 envelope glycoprotein gp120. Proceedings of the National Academy of Sciences of the United States of America, 88(16), 7131-7134. https://doi.org/10.1073/pnas.88.16.7131

Galactosyl ceramide or a derivative is an essential component of the neural receptor for human immunodeficiency virus type 1 envelope glycoprotein gp120. / Bhat, Shama; Spitalnik, Steven L.; Gonzalez-Scarano, Francisco; Silberberg, Donald H.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, No. 16, 1991, p. 7131-7134.

Research output: Contribution to journal › Article › peer-review

Bhat, S, Spitalnik, SL, Gonzalez-Scarano, F & Silberberg, DH 1991, 'Galactosyl ceramide or a derivative is an essential component of the neural receptor for human immunodeficiency virus type 1 envelope glycoprotein gp120', Proceedings of the National Academy of Sciences of the United States of America, vol. 88, no. 16, pp. 7131-7134. https://doi.org/10.1073/pnas.88.16.7131

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abstract = "This report demonstrates that galactosyl ceramide (GalCer) or a molecule derived from it may serve as an alternative receptor for human immunodeficiency virus in the nervous system. Recombinant gp120, an envelope glycoprotein of human immunodeficiency virus type 1, specifically binds to GalCer and its derivatives. This specificity was studied by inhibiting binding of radioiodinated gp120 to GalCer with antibodies to GalCer, antibodies to gp120, and an excess of unlabeled gp120. Binding activity was also removed by absorbing gp120 with liposomes containing GalCer. In addition, studies using natural and semisynthetic lipids indicate that the linkage between galactose and ceramide is essential for binding. The significance of an alternative receptor for human immunodeficiency virus in the nervous system is discussed.",

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Galactosyl ceramide or a derivative is an essential component of the neural receptor for human immunodeficiency virus type 1 envelope glycoprotein gp120

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