Abstract
Gβ5 exists as two splice variants, Gβ5-S and Gβ5-L, which interact with and stabilize the R7 members of the regulators of G-protein signaling (RGSs): RGS6, RGS7, RGS9, and RGS11. Although the role of Gβ5-L and RGS9-1 is established in photoreceptors, the physiological functions of Gβ5-S and other R7 RGS proteins remain unclear. We found that the electroretinogram of Gβ5-/- mice lacks the b-wave component and that Gβ5-S and RGS11 colocalize with Goα at the tips of the ON-bipolar cell dendrites. Unexpectedly, we found a significant reduction in the number of synaptic triads in the outer plexiform layer (OPL) of the Gβ5-/- mice, which is evident at postnatal day 14. Transgenic expression of Gβ5-L in rods failed to rescue the b-wave or the OPL defects. These results indicate that Gβ5-S is indispensable for OPL integrity and normal light responses of the retina.
Original language | English (US) |
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Pages (from-to) | 14199-14204 |
Number of pages | 6 |
Journal | Journal of Neuroscience |
Volume | 27 |
Issue number | 51 |
DOIs | |
State | Published - Dec 19 2007 |
Externally published | Yes |
Keywords
- Bipolar cells
- ERG
- Gβ5
- Morphology
- RGS
- Retina
ASJC Scopus subject areas
- General Neuroscience