Further studies of salivary inhibition of hiv-1 infectivity

Chih Ko Yeh, Beverly Handelman, Philip C. Fox, Bruce J. Baum

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

An HIV-l/ATH8-cell cytopathic system was used to characterize the previously reported anti-HIV-1 activity of human saliva. Inhibitory activity was demonstrated by monitoring viable cell counts, HIV-1 p24 core antigen, and reverse transcriptase levels. Nonfiltered whole saliva, sterilized by irradiation, protected the ATH8 cells from HIV-1 infection. When HIV-1/saliva mixtures were filtered following incubation, the quantity of virus was significantly less (˜50%) than in HIV-1/media-filtered controls, suggesting that salivary aggregation and/or agglutination may be involved in the inhibitory activity. However, a sufficient number of apparently morphologically intact viral particles were still present in the HIV-1/saliva filtrates to lead to infection. When saliva was filtered prior to incubation with HIV-1, these filtrates showed substantial inhibitory activity, although reduced compared with that of non-prefiltered saliva. We conclude that saliva likely has several means by which to inhibit HIV-1 infectivity.

Original languageEnglish (US)
Pages (from-to)898-903
Number of pages6
JournalJournal of Acquired Immune Deficiency Syndromes
Volume5
Issue number9
StatePublished - Sep 1992
Externally publishedYes

Keywords

  • ATH8 cell
  • Antiviral
  • Cytopathy
  • HIV-1
  • Saliva

ASJC Scopus subject areas

  • Infectious Diseases
  • Pharmacology (medical)

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