Abstract
The collagenase B type IV (Co14B) gene is highly expressed in the osteoclast, the primary bone-resorbing cell. However, factors that regulate expression of the Co14B gene are not well characterized. A murine P1 genomic clone containing a 94 kb sequence insert which contains the Co14B gene was isolated. A 4 kb EcoR1 DNA fragment containing the 5' flanking sequence of the gene was further subcloned and restriction mapped. Putative transcription factors such as SRY, Lyf-1, and GATA1 and 2, binding motifs were identified by sequence analysis in this promoter region. Enhancer and suppressor regions were mapped by transient expression of Co14B gene promoter deletion mutant-luciferase reporter gene constructs in HepG2 cells. Co14B mRNA expression in different murine tissues was analyzed by reverse transcription-polymerase chain reaction and demonstrated high levels of expression in bone, clavaria, spleen and thymus. This promoter provides a valuable tool for targeting gene expression to the osteoclast.
Original language | English (US) |
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Pages (from-to) | 117-122 |
Number of pages | 6 |
Journal | Gene |
Volume | 208 |
Issue number | 2 |
DOIs | |
State | Published - Feb 22 1998 |
Keywords
- Collagenase
- Osteoclast
- Promoter
- Transcription factor
ASJC Scopus subject areas
- Genetics