Further analysis of the CAP59 locus of Cryptococcus neoformans: structure defined by forced expression and description of a new ribosomal protein-encoding gene

Yun C. Chang, B. L. Wickes, K. J. Kwon-Chung

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Cryptococcus neoformans (Cn) produces an extracellular polysaccharide capsule that is an essential factor for virulence. We previously isolated a gene, CAP59, which is necessary for capsule formation. To dissect the functional region of CAP59, we placed it under control of the Cn GAL7 promoter (pGAL7). Among the several pGAL7::CAP59 fusion constructs, only the one containing the entire open reading frame of CAP59 was able to complement the acapsular phenotype under galactose induction. A missense mutation in the coding region abolished complementation by the fusion construct. We also found that the CAP59 locus is contiguous to a convergently transcribed L27 ribosomal protein-encoding gene (CL27). The distance between the cDNA ends of these two genes is only 25 bp. CL27 has two introns near its N terminus. The translated CL27 protein is 183 amino acids (aa) in length with an estimated molecular mass of 20 kDa, and the first 34 aa at the N terminus may be a targeting peptide for mitochondria. A high degree of restriction-fragment-length polymorphism was detected in the DNA sequence containing CAP59 and CL27.

Original languageEnglish (US)
Pages (from-to)179-183
Number of pages5
JournalGene
Volume167
Issue number1-2
DOIs
Publication statusPublished - Dec 29 1995

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Keywords

  • Capsule
  • GAL7
  • L27 ribosomal protein
  • fungus
  • polysaccharide
  • transmembrane protein
  • virulence

ASJC Scopus subject areas

  • Genetics

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