TY - JOUR
T1 - Functionality of unliganded VDR in breast cancer cells
T2 - Repressive action on CYP24 basal transcription
AU - Alimirah, Fatouma
AU - Vaishnav, Avani
AU - McCormick, Michael
AU - Echchgadda, Ibtissam
AU - Chatterjee, Bandana
AU - Mehta, Rajendra G.
AU - Peng, Xinjian
N1 - Funding Information:
Acknowledgments This study was supported by NCI Public Health Service Grant CA 82316 (R. G. M.) and NCI R03 CA121365-02 (X. P.). F.A. is supported by the Ruth L. Kirschstein predoctoral fellowship (1F31CA13261903). B.A is a VA research Career Scientist and is supported by a VA Merit-Review grant.
PY - 2010/9
Y1 - 2010/9
N2 - It is well-established that CYP24, an immediate target gene of VDR is upregulated by VDR ligands. This study is focused on the functional role of unliganded VDR by investigating the correlation between the expression of VDR protein and basal mRNA levels of CYP24 in breast cancer cell lines. Analyses of multiple breast cancer cell lines demonstrated an inverse correlation between VDR protein expression and CYP24 mRNA expression levels; while in the presence of ligand, VDR protein level was positively correlated with CYP24 expression. In MCF-7 cells, VDR was mainly distributed in the nuclei in the absence of ligand. VDR overexpression in MCF-7 cells and MDA-MB231 cells decreased CYP24 mRNA expression levels and CYP24 promoter activity. Conversely, knock-down of VDR using siRNA techniques in MCF-7 and T47D cells significantly increased CYP24 mRNA expression. We also found that overexpression of VDR with a polymorphic site (FokI-FF) at its AF-1 domain, which makes VDR shorter by three amino acids, failed to repress CYP24 promoter activity. This report provides conclusive evidence for the repressive action of unliganded VDR on the expression of its target gene CYP24 and the importance of an intact VDR AF-1 domain for its repressive action.
AB - It is well-established that CYP24, an immediate target gene of VDR is upregulated by VDR ligands. This study is focused on the functional role of unliganded VDR by investigating the correlation between the expression of VDR protein and basal mRNA levels of CYP24 in breast cancer cell lines. Analyses of multiple breast cancer cell lines demonstrated an inverse correlation between VDR protein expression and CYP24 mRNA expression levels; while in the presence of ligand, VDR protein level was positively correlated with CYP24 expression. In MCF-7 cells, VDR was mainly distributed in the nuclei in the absence of ligand. VDR overexpression in MCF-7 cells and MDA-MB231 cells decreased CYP24 mRNA expression levels and CYP24 promoter activity. Conversely, knock-down of VDR using siRNA techniques in MCF-7 and T47D cells significantly increased CYP24 mRNA expression. We also found that overexpression of VDR with a polymorphic site (FokI-FF) at its AF-1 domain, which makes VDR shorter by three amino acids, failed to repress CYP24 promoter activity. This report provides conclusive evidence for the repressive action of unliganded VDR on the expression of its target gene CYP24 and the importance of an intact VDR AF-1 domain for its repressive action.
KW - Breast cancer cells
KW - CYP24
KW - Unliganded VDR
KW - Vitamin D receptor
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U2 - 10.1007/s11010-010-0478-6
DO - 10.1007/s11010-010-0478-6
M3 - Article
C2 - 20440542
AN - SCOPUS:77955853645
SN - 0300-8177
VL - 342
SP - 143
EP - 150
JO - Molecular and Cellular Biochemistry
JF - Molecular and Cellular Biochemistry
IS - 1-2
ER -