Functional role of M-type (KCNQ) K+ channels in adrenergic control of cardiomyocyte contraction rate by sympathetic neurons

Oleg Zaika, Jie Zhang, Mark S Shapiro

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

M-type (KCNQ) K+ channels are known to regulate excitability and firing properties of sympathetic neurons (SNs), but their role in regulating neurotransmitter release is unclear, requiring further study. We sought to use a physiological preparation in which SNs innervate primary cardiomyocytes to evaluate the direct role of M-channels in the release of noradrenaline (NA) from SNs. Co-cultures of rat SNs and mouse cardiomyocytes were prepared, and the contraction rate (CR) of the cardiomyocyte syncytium monitored by video microscopy. We excited the SNs with nicotine, acting on nicotinic acetylcholine receptors, and monitored the increase in CR in the presence or absence of the specific M-channel opener retigabine, or agonists of bradykinin B2 or purinergic P2Y receptors on the SNs. The maximal adrenergic effect on the CR was determined by application of isoproterenol (isoprenaline). To isolate the actions of B2 or P2Y receptor stimulation to the neurons, we prepared cardiomyocytes from B2 receptor or P2Y2 receptor knock-out mice, respectively. We found that co-application of retigabine strongly decreased the nicotine-induced increase in CR. Conversely, co-application of bradykinin or the P2Y-receptor agonist UTP augmented the nicotine-induced increase in CR to about half of the level produced by isoproterenol. All effects on the CR were wholly blocked by propranolol. Our data support the role of M-type K+ channels in the control of NA release by SNs at functional adrenergic synapses on cardiomyocytes. We conclude that physiological receptor agonists control the heart rate via the regulation of M-current in SNs.

Original languageEnglish (US)
Pages (from-to)2559-2568
Number of pages10
JournalJournal of Physiology
Volume589
Issue number10
DOIs
StatePublished - May 2011

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Cardiac Myocytes
Adrenergic Agents
Neurons
Nicotine
Isoproterenol
Bradykinin
Norepinephrine
Purinergic P2Y Receptors
Purinergic P2Y2 Receptors
Video Microscopy
Nicotinic Receptors
Giant Cells
Coculture Techniques
Knockout Mice
Propranolol
Synapses
Neurotransmitter Agents
Heart Rate

ASJC Scopus subject areas

  • Physiology

Cite this

Functional role of M-type (KCNQ) K+ channels in adrenergic control of cardiomyocyte contraction rate by sympathetic neurons. / Zaika, Oleg; Zhang, Jie; Shapiro, Mark S.

In: Journal of Physiology, Vol. 589, No. 10, 05.2011, p. 2559-2568.

Research output: Contribution to journalArticle

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