Functional role of AMP-activated protein kinase in the heart during exercise

Nicolas Musi; Michael F. Hirshman; Michael Arad; Yanqiu Xing; Nobuharu Fujii; Jason Pomerleau; Ferhaan Ahmad; Charles I. Berul; Jon G. Seidman; Rong Tian; Laurie J. Goodyear

doi:10.1016/j.febslet.2005.02.052

Functional role of AMP-activated protein kinase in the heart during exercise

Nicolas Musi, Michael F. Hirshman, Michael Arad, Yanqiu Xing, Nobuharu Fujii, Jason Pomerleau, Ferhaan Ahmad, Charles I. Berul, Jon G. Seidman, Rong Tian, Laurie J. Goodyear

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

AMP-activated protein kinase (AMPK) plays a critical role in maintaining energy homeostasis and cardiac function during ischemia in the heart. However, the functional role of AMPK in the heart during exercise is unknown. We examined whether acute exercise increases AMPK activity in mouse hearts and determined the significance of these increases by studying transgenic (TG) mice expressing a cardiac-specific dominant-negative (inactivating) AMPKα2 subunit. Exercise increased cardiac AMPKα2 activity in the wild type mice but not in TG. We found that inactivation of AMPK did not result in abnormal ATP and glycogen consumption during exercise, cardiac function assessed by heart rhythm telemetry and stress echocardiography, or in maximal exercise capacity.

Original language	English (US)
Pages (from-to)	2045-2050
Number of pages	6
Journal	FEBS Letters
Volume	579
Issue number	10
DOIs	https://doi.org/10.1016/j.febslet.2005.02.052
State	Published - Apr 11 2005
Externally published	Yes

Keywords

AMP-activated protein kinase
Acetyl CoA carboxylase
Exercise
Heart
p38

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2005.02.052

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title = "Functional role of AMP-activated protein kinase in the heart during exercise",

abstract = "AMP-activated protein kinase (AMPK) plays a critical role in maintaining energy homeostasis and cardiac function during ischemia in the heart. However, the functional role of AMPK in the heart during exercise is unknown. We examined whether acute exercise increases AMPK activity in mouse hearts and determined the significance of these increases by studying transgenic (TG) mice expressing a cardiac-specific dominant-negative (inactivating) AMPKα2 subunit. Exercise increased cardiac AMPKα2 activity in the wild type mice but not in TG. We found that inactivation of AMPK did not result in abnormal ATP and glycogen consumption during exercise, cardiac function assessed by heart rhythm telemetry and stress echocardiography, or in maximal exercise capacity.",

keywords = "AMP-activated protein kinase, Acetyl CoA carboxylase, Exercise, Heart, p38",

author = "Nicolas Musi and Hirshman, {Michael F.} and Michael Arad and Yanqiu Xing and Nobuharu Fujii and Jason Pomerleau and Ferhaan Ahmad and Berul, {Charles I.} and Seidman, {Jon G.} and Rong Tian and Goodyear, {Laurie J.}",

note = "Funding Information: This work was supported by Grants AR45670 (L.J.G.) and HL67970 (R.T.) from the National Institutes of Health. The work was also supported by an Established Investigator Award from the American Heart Association to Dr. Tian and an American Diabetes Association Mentor Based Award to Dr. Goodyear. We also thank Laura E. Holton for excellent technical assistance. ",

year = "2005",

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day = "11",

doi = "10.1016/j.febslet.2005.02.052",

language = "English (US)",

volume = "579",

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TY - JOUR

T1 - Functional role of AMP-activated protein kinase in the heart during exercise

AU - Musi, Nicolas

AU - Hirshman, Michael F.

AU - Arad, Michael

AU - Xing, Yanqiu

AU - Fujii, Nobuharu

AU - Pomerleau, Jason

AU - Ahmad, Ferhaan

AU - Berul, Charles I.

AU - Seidman, Jon G.

AU - Tian, Rong

AU - Goodyear, Laurie J.

N1 - Funding Information: This work was supported by Grants AR45670 (L.J.G.) and HL67970 (R.T.) from the National Institutes of Health. The work was also supported by an Established Investigator Award from the American Heart Association to Dr. Tian and an American Diabetes Association Mentor Based Award to Dr. Goodyear. We also thank Laura E. Holton for excellent technical assistance.

PY - 2005/4/11

Y1 - 2005/4/11

N2 - AMP-activated protein kinase (AMPK) plays a critical role in maintaining energy homeostasis and cardiac function during ischemia in the heart. However, the functional role of AMPK in the heart during exercise is unknown. We examined whether acute exercise increases AMPK activity in mouse hearts and determined the significance of these increases by studying transgenic (TG) mice expressing a cardiac-specific dominant-negative (inactivating) AMPKα2 subunit. Exercise increased cardiac AMPKα2 activity in the wild type mice but not in TG. We found that inactivation of AMPK did not result in abnormal ATP and glycogen consumption during exercise, cardiac function assessed by heart rhythm telemetry and stress echocardiography, or in maximal exercise capacity.

AB - AMP-activated protein kinase (AMPK) plays a critical role in maintaining energy homeostasis and cardiac function during ischemia in the heart. However, the functional role of AMPK in the heart during exercise is unknown. We examined whether acute exercise increases AMPK activity in mouse hearts and determined the significance of these increases by studying transgenic (TG) mice expressing a cardiac-specific dominant-negative (inactivating) AMPKα2 subunit. Exercise increased cardiac AMPKα2 activity in the wild type mice but not in TG. We found that inactivation of AMPK did not result in abnormal ATP and glycogen consumption during exercise, cardiac function assessed by heart rhythm telemetry and stress echocardiography, or in maximal exercise capacity.

KW - AMP-activated protein kinase

KW - Acetyl CoA carboxylase

KW - Exercise

KW - Heart

KW - p38

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SN - 0014-5793

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EP - 2050

JO - FEBS Letters

JF - FEBS Letters

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Functional role of AMP-activated protein kinase in the heart during exercise

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Keywords

ASJC Scopus subject areas

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