Functional reconstitution of the human epithelial Na+ channel in a mammalian expression system.

Alexander Staruschenko, Rachell E. Booth, Oleh Pochynyuk, James D. Stockand, Qiusheng Tong

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Probing ion channel structure-function and regulation in native tissue can, in some instances, be experimentally challenging or impractical. To facilitate discovery and increase experimental flexibility, our laboratory routinely reconstitutes recombinant ion channels in a mammalian expression system quantifying channel activity with patch clamp electrophysiology. Here, we describe investigation of the human epithelial Na+ channel heterologously expressed in Chinese hamster ovary cells.

Original languageEnglish (US)
Pages (from-to)3-13
Number of pages11
JournalMethods in molecular biology (Clifton, N.J.)
Volume337
DOIs
StatePublished - 2006

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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