Functional reconstitution of the human epithelial Na+ channel in a mammalian expression system.

Alexander Staruschenko, Rachell E. Booth, Oleh Pochynyuk, James D Stockand, Qiusheng Tong

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Probing ion channel structure-function and regulation in native tissue can, in some instances, be experimentally challenging or impractical. To facilitate discovery and increase experimental flexibility, our laboratory routinely reconstitutes recombinant ion channels in a mammalian expression system quantifying channel activity with patch clamp electrophysiology. Here, we describe investigation of the human epithelial Na+ channel heterologously expressed in Chinese hamster ovary cells.

Original languageEnglish (US)
Pages (from-to)3-13
Number of pages11
JournalMethods in molecular biology (Clifton, N.J.)
Volume337
StatePublished - 2006

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Epithelial Sodium Channels
Ion Channels
Electrophysiology
Cricetulus
Ovary

ASJC Scopus subject areas

  • Medicine(all)

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Functional reconstitution of the human epithelial Na+ channel in a mammalian expression system. / Staruschenko, Alexander; Booth, Rachell E.; Pochynyuk, Oleh; Stockand, James D; Tong, Qiusheng.

In: Methods in molecular biology (Clifton, N.J.), Vol. 337, 2006, p. 3-13.

Research output: Contribution to journalArticle

Staruschenko, Alexander ; Booth, Rachell E. ; Pochynyuk, Oleh ; Stockand, James D ; Tong, Qiusheng. / Functional reconstitution of the human epithelial Na+ channel in a mammalian expression system. In: Methods in molecular biology (Clifton, N.J.). 2006 ; Vol. 337. pp. 3-13.
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