Functional expression of brain cholecystokinin and bombesin receptors in Xenopus oocytes

Thomas M. Moriarty, Boaz Gillo, Stuart Sealfon, James L. Roberts, Robert D. Blitzer, Emmanuel M. Landau

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Total RNA was extracted from 15-day-old whole rat brains. Microinjection of the RNA into Xenopus laevis oocytes induced electrophysiological responsiveness to cholecystokinin-8 (CCK) and bombesin (BBS) but not to corticotropin-releasing factor (CRF) or somatostatin. The responses to CCK and BBS were similar in shape, time course, and reversal potential to that induced by receptor mediated phospholipid breakdown and that which is induced by intracellular injection of IP3. These responses were not blocked by atropine or by mianserin, did not require extracellular Ca2+ and were completely suppressed by intracellular injection of EGTA.

Original languageEnglish (US)
Pages (from-to)75-79
Number of pages5
JournalMolecular Brain Research
Volume4
Issue number1
DOIs
StatePublished - Aug 1988
Externally publishedYes

Keywords

  • Bombesin receptor
  • Cholecystokinin receptor
  • In vivo translation
  • Microinjection of brain RNA
  • Neuropeptide
  • Xenopus oocyte

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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