TY - JOUR
T1 - Functional cross-talk among Rad51, Rad54, and replication protein A in heteroduplex DNA joint formation
AU - Van Komen, Stephen
AU - Petukhova, Galina
AU - Sigurdsson, Stefan
AU - Sung, Patrick
PY - 2002/11/15
Y1 - 2002/11/15
N2 - Saccharomyces cerevisiae Rad51, Rad54, and replication protein A (RPA) proteins work in concert to make heteroduplex DNA joints during homologous recombination. With plasmid length DNA substrates, maximal DNA joint formation is observed with amounts of Rad51 substantially below what is needed to saturate the initiating single-stranded DNA template, and, relative to Rad51, Rad54 is needed in only catalytic quantities. RPA is still indispensable for optimal reaction efficiency, but its role in this instance is to sequester free single-stranded DNA, which otherwise inhibits Rad51 and Rad54 functions. We also demonstrate that Rad54 helps overcome various reaction constraints in DNA joint formation. These results thus shed light on the function of Rad54 in the Rad51-mediated homologous DNA pairing reaction and also reveal a novel role of RPA in the presynaptic stage of this reaction.
AB - Saccharomyces cerevisiae Rad51, Rad54, and replication protein A (RPA) proteins work in concert to make heteroduplex DNA joints during homologous recombination. With plasmid length DNA substrates, maximal DNA joint formation is observed with amounts of Rad51 substantially below what is needed to saturate the initiating single-stranded DNA template, and, relative to Rad51, Rad54 is needed in only catalytic quantities. RPA is still indispensable for optimal reaction efficiency, but its role in this instance is to sequester free single-stranded DNA, which otherwise inhibits Rad51 and Rad54 functions. We also demonstrate that Rad54 helps overcome various reaction constraints in DNA joint formation. These results thus shed light on the function of Rad54 in the Rad51-mediated homologous DNA pairing reaction and also reveal a novel role of RPA in the presynaptic stage of this reaction.
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U2 - 10.1074/jbc.M205864200
DO - 10.1074/jbc.M205864200
M3 - Article
C2 - 12226081
AN - SCOPUS:0037113947
SN - 0021-9258
VL - 277
SP - 43578
EP - 43587
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 46
ER -