Abstract
1. Administration of 2-methyl-4-dimethylaminobenzene in the diet (0.1%, w/w) for 85-90 days doubled the content of mitochondria in the livers of rats. The azodye was covalently bound to liver proteins, and about 15% of the amount found in liver was associated with the mitochondrial fraction. 2. Mitochondria isolated from the livers of azodye-fed animals showed drastically lowered ability to oxidize NAD+-linked substrates. The inhibted electron-transfer step was the reduction of ubiquinone. The organelles showed a large increase in succinate oxidase activity. 3. The activity of cytochrome oxidase and the content of cytochrome aa3 were substantially higher in these organelles. 4. Azodye-fed animals showed depressed serum cholesterol concentrations. The content of ubiquinone in liver also registered a small increase.
Original language | English (US) |
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Pages (from-to) | 955-960 |
Number of pages | 6 |
Journal | Biochemical Journal |
Volume | 224 |
Issue number | 3 |
DOIs | |
State | Published - 1984 |
Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology