Functional changes in rat liver mitochondria on administration of 2-methyl-4-dimethylaminoazobenzene

P. Saikumar, C. K. Ramakrishna Kurup

Research output: Contribution to journalArticle

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Abstract

1. Administration of 2-methyl-4-dimethylaminobenzene in the diet (0.1%, w/w) for 85-90 days doubled the content of mitochondria in the livers of rats. The azodye was covalently bound to liver proteins, and about 15% of the amount found in liver was associated with the mitochondrial fraction. 2. Mitochondria isolated from the livers of azodye-fed animals showed drastically lowered ability to oxidize NAD+-linked substrates. The inhibted electron-transfer step was the reduction of ubiquinone. The organelles showed a large increase in succinate oxidase activity. 3. The activity of cytochrome oxidase and the content of cytochrome aa3 were substantially higher in these organelles. 4. Azodye-fed animals showed depressed serum cholesterol concentrations. The content of ubiquinone in liver also registered a small increase.

Original languageEnglish (US)
Pages (from-to)955-960
Number of pages6
JournalBiochemical Journal
Volume224
Issue number3
DOIs
StatePublished - Jan 1 1984
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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