Functional aspects of eicosanoid hydroxylation by lung and kidney cytochromes P450. Expression of cDNAs in mammalian cells and E. coli

B. S S Masters, J. E. Clark, L. J. Roman, M. Nishimoto, T. J. McCabe, P. R. Ortiz De Montellano, C. G. Plopper, D. Gebremedhin, Y. H. Ma, D. R. Harder, R. J. Roman

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A gene subfamily of cytochromes P450 with catalytic activity toward various eicosanoid substrates has been studied with a variety of techniques in this laboratory, including purification and characterization, localization at the tissue and subcellular levels, physiological function, and cloning and expression in prokaryotic and eukaryotic systems. This paper reports experiments directed toward determining the function of the cytochrome P4504A metabolite, 20-hydroxyarachidonic acid (20-hydroxyeicosatetraenoic acid; 20-HETE), in cellular ion flux, immunohistochemical localization in lung, the effects of a mechanism-based inhibitor, 12-hydroxy-16-heptadecynoic acid (12-HHDYA) on PGE1 ω-hydroxylation, and the structure-function determinants which govern the activities of the enzymes encoded by this gene subfamily.

Original languageEnglish (US)
Pages (from-to)353-360
Number of pages8
JournalJournal of Lipid Mediators
Volume6
Issue number1-3
StatePublished - 1992

Fingerprint

Hydroxylation
Eicosanoids
Cytochrome P-450 Enzyme System
Escherichia coli
Complementary DNA
Cells
Kidney
Lung
Alprostadil
Cytochromes
Genes
Organism Cloning
Cloning
Ions
Metabolites
Acids
Purification
Catalyst activity
Enzymes
Tissue

Keywords

  • ω-hydroxylation
  • 20-hydroxyarachidonate
  • chimeric constructs
  • COS-1 cell expression
  • CYP4A gene subfamily
  • cytochromes P4504A
  • E. coli-expressed P4504A4
  • eicosanoids
  • immunohistochemical localization
  • mechanism-based inhibition
  • patch-clamp study

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Cite this

Masters, B. S. S., Clark, J. E., Roman, L. J., Nishimoto, M., McCabe, T. J., Ortiz De Montellano, P. R., ... Roman, R. J. (1992). Functional aspects of eicosanoid hydroxylation by lung and kidney cytochromes P450. Expression of cDNAs in mammalian cells and E. coli. Journal of Lipid Mediators, 6(1-3), 353-360.

Functional aspects of eicosanoid hydroxylation by lung and kidney cytochromes P450. Expression of cDNAs in mammalian cells and E. coli. / Masters, B. S S; Clark, J. E.; Roman, L. J.; Nishimoto, M.; McCabe, T. J.; Ortiz De Montellano, P. R.; Plopper, C. G.; Gebremedhin, D.; Ma, Y. H.; Harder, D. R.; Roman, R. J.

In: Journal of Lipid Mediators, Vol. 6, No. 1-3, 1992, p. 353-360.

Research output: Contribution to journalArticle

Masters, BSS, Clark, JE, Roman, LJ, Nishimoto, M, McCabe, TJ, Ortiz De Montellano, PR, Plopper, CG, Gebremedhin, D, Ma, YH, Harder, DR & Roman, RJ 1992, 'Functional aspects of eicosanoid hydroxylation by lung and kidney cytochromes P450. Expression of cDNAs in mammalian cells and E. coli', Journal of Lipid Mediators, vol. 6, no. 1-3, pp. 353-360.
Masters BSS, Clark JE, Roman LJ, Nishimoto M, McCabe TJ, Ortiz De Montellano PR et al. Functional aspects of eicosanoid hydroxylation by lung and kidney cytochromes P450. Expression of cDNAs in mammalian cells and E. coli. Journal of Lipid Mediators. 1992;6(1-3):353-360.
Masters, B. S S ; Clark, J. E. ; Roman, L. J. ; Nishimoto, M. ; McCabe, T. J. ; Ortiz De Montellano, P. R. ; Plopper, C. G. ; Gebremedhin, D. ; Ma, Y. H. ; Harder, D. R. ; Roman, R. J. / Functional aspects of eicosanoid hydroxylation by lung and kidney cytochromes P450. Expression of cDNAs in mammalian cells and E. coli. In: Journal of Lipid Mediators. 1992 ; Vol. 6, No. 1-3. pp. 353-360.
@article{2b3aceaf157a424d803c838313f7865e,
title = "Functional aspects of eicosanoid hydroxylation by lung and kidney cytochromes P450. Expression of cDNAs in mammalian cells and E. coli",
abstract = "A gene subfamily of cytochromes P450 with catalytic activity toward various eicosanoid substrates has been studied with a variety of techniques in this laboratory, including purification and characterization, localization at the tissue and subcellular levels, physiological function, and cloning and expression in prokaryotic and eukaryotic systems. This paper reports experiments directed toward determining the function of the cytochrome P4504A metabolite, 20-hydroxyarachidonic acid (20-hydroxyeicosatetraenoic acid; 20-HETE), in cellular ion flux, immunohistochemical localization in lung, the effects of a mechanism-based inhibitor, 12-hydroxy-16-heptadecynoic acid (12-HHDYA) on PGE1 ω-hydroxylation, and the structure-function determinants which govern the activities of the enzymes encoded by this gene subfamily.",
keywords = "ω-hydroxylation, 20-hydroxyarachidonate, chimeric constructs, COS-1 cell expression, CYP4A gene subfamily, cytochromes P4504A, E. coli-expressed P4504A4, eicosanoids, immunohistochemical localization, mechanism-based inhibition, patch-clamp study",
author = "Masters, {B. S S} and Clark, {J. E.} and Roman, {L. J.} and M. Nishimoto and McCabe, {T. J.} and {Ortiz De Montellano}, {P. R.} and Plopper, {C. G.} and D. Gebremedhin and Ma, {Y. H.} and Harder, {D. R.} and Roman, {R. J.}",
year = "1992",
language = "English (US)",
volume = "6",
pages = "353--360",
journal = "Prostaglandins and Other Lipid Mediators",
issn = "0090-6980",
publisher = "Elsevier BV",
number = "1-3",

}

TY - JOUR

T1 - Functional aspects of eicosanoid hydroxylation by lung and kidney cytochromes P450. Expression of cDNAs in mammalian cells and E. coli

AU - Masters, B. S S

AU - Clark, J. E.

AU - Roman, L. J.

AU - Nishimoto, M.

AU - McCabe, T. J.

AU - Ortiz De Montellano, P. R.

AU - Plopper, C. G.

AU - Gebremedhin, D.

AU - Ma, Y. H.

AU - Harder, D. R.

AU - Roman, R. J.

PY - 1992

Y1 - 1992

N2 - A gene subfamily of cytochromes P450 with catalytic activity toward various eicosanoid substrates has been studied with a variety of techniques in this laboratory, including purification and characterization, localization at the tissue and subcellular levels, physiological function, and cloning and expression in prokaryotic and eukaryotic systems. This paper reports experiments directed toward determining the function of the cytochrome P4504A metabolite, 20-hydroxyarachidonic acid (20-hydroxyeicosatetraenoic acid; 20-HETE), in cellular ion flux, immunohistochemical localization in lung, the effects of a mechanism-based inhibitor, 12-hydroxy-16-heptadecynoic acid (12-HHDYA) on PGE1 ω-hydroxylation, and the structure-function determinants which govern the activities of the enzymes encoded by this gene subfamily.

AB - A gene subfamily of cytochromes P450 with catalytic activity toward various eicosanoid substrates has been studied with a variety of techniques in this laboratory, including purification and characterization, localization at the tissue and subcellular levels, physiological function, and cloning and expression in prokaryotic and eukaryotic systems. This paper reports experiments directed toward determining the function of the cytochrome P4504A metabolite, 20-hydroxyarachidonic acid (20-hydroxyeicosatetraenoic acid; 20-HETE), in cellular ion flux, immunohistochemical localization in lung, the effects of a mechanism-based inhibitor, 12-hydroxy-16-heptadecynoic acid (12-HHDYA) on PGE1 ω-hydroxylation, and the structure-function determinants which govern the activities of the enzymes encoded by this gene subfamily.

KW - ω-hydroxylation

KW - 20-hydroxyarachidonate

KW - chimeric constructs

KW - COS-1 cell expression

KW - CYP4A gene subfamily

KW - cytochromes P4504A

KW - E. coli-expressed P4504A4

KW - eicosanoids

KW - immunohistochemical localization

KW - mechanism-based inhibition

KW - patch-clamp study

UR - http://www.scopus.com/inward/record.url?scp=0026969309&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026969309&partnerID=8YFLogxK

M3 - Article

C2 - 8357994

AN - SCOPUS:0026969309

VL - 6

SP - 353

EP - 360

JO - Prostaglandins and Other Lipid Mediators

JF - Prostaglandins and Other Lipid Mediators

SN - 0090-6980

IS - 1-3

ER -