Function of von Willebrand factor after crossed bone marrow transplantation between normal and von Willebrand disease pigs: Effect on arterial thrombosis in chimeras

Timothy C. Nichols, C. Marc Samama, Dwight A. Bellinger, Jacqueline Roussi, Robert L. Reddick, Michel Bonneau, Marjorie S. Read, O. Bailliart, Gary G. Koch, Marcel Vaiman, Jeff L. Sigman, George A. Pignaud, Kenneth M. Brinkhous, Thomas R. Griggs, Ludovic Drouet

Research output: Contribution to journalArticlepeer-review

79 Scopus citations

Abstract

von Willebrand factor (vWF) is essential for the induction of occlusive thrombosis in stenosed and injured pig arteries and for normal hemostasis. To separate the relative contribution of plasma and platelet vWF to arterial thrombosis, we produced chimeric normal and von Willebrand disease pigs by crossed bone marrow transplantation; von Willebrand disease (vWD) pigs were engrafted with normal pig bone marrow and normal pigs were engrafted with vWD bone marrow. Thrombosis developed in the chimeric normal pigs that showed normal levels of plasma vWF and an absence of platelet vWF; but no thrombosis occurred in the chimeric vWD pigs that demonstrated normal platelet vWF and an absence of plasma vWF. The ear bleeding times of the chimeric pigs were partially corrected by endogenous plasma vWF but not by platelet vWF. Our animal model demonstrated that vWF in the plasma compartment is essential for the development of arterial thrombosis and that it also contributes to the maintenance of bleeding time and hemostasis.

Original languageEnglish (US)
Pages (from-to)2455-2459
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number7
DOIs
StatePublished - Mar 28 1995
Externally publishedYes

Keywords

  • animal model
  • chimerism
  • factor VIII
  • platelets

ASJC Scopus subject areas

  • General

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