Freshly isolated mouse 4F7+ splenic dendritic cells process and present exogenous antigens to T cells

Mansour Mohamadzadeh, Anastassia Pavlidou, Alexander Enk, Jürgen Knop, Erwin Rüde, Gernot Gradehandt

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


The antibody 4F7 was reported to recognize an epitope expressed on dendritic cells (DC) from various tissues. To study the ability of splenic 4F7+ dendritic cells to process antigen for presentation to CD4+ T cells, DC were enriched using a separation procedure avoiding overnight culture which could lead to an altered phenotype. These DC were used as antigen‐presenting cells (APC) in stimulation cultures of major histocompatibility complex class II‐restricted T cells. It was found that they induce antigen‐dependent lymphokine production by T cells and therefore could present exogenous antigens. These processing takes place intracellularly, because fixation abrogates presentation to T cells. Moreover, antigen presentation needs intracellular processing within endo‐ or lysosomes as chloroquine‐treatment prevents T cell activation. Titration of APC numbers revealed that contaminating APC most likely did not account for antigen‐specific T cell activation by DC. No evidence was found for release of antigenic peptides or for partial antigen processing possibly done by cell surface located enzymes on DC. In conclusion, these results indicate that freshly enriched DC are able to process antigens similarly to other APC.

Original languageEnglish (US)
Pages (from-to)3170-3174
Number of pages5
JournalEuropean Journal of Immunology
Issue number12
StatePublished - Dec 1994
Externally publishedYes


  • Antigen presentation
  • Antigen processing
  • CD4 T cells
  • Dendritic cell
  • Mice

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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