TY - JOUR
T1 - Freshly isolated mouse 4F7+ splenic dendritic cells process and present exogenous antigens to T cells
AU - Mohamadzadeh, Mansour
AU - Pavlidou, Anastassia
AU - Enk, Alexander
AU - Knop, Jürgen
AU - Rüde, Erwin
AU - Gradehandt, Gernot
PY - 1994/12
Y1 - 1994/12
N2 - The antibody 4F7 was reported to recognize an epitope expressed on dendritic cells (DC) from various tissues. To study the ability of splenic 4F7+ dendritic cells to process antigen for presentation to CD4+ T cells, DC were enriched using a separation procedure avoiding overnight culture which could lead to an altered phenotype. These DC were used as antigen‐presenting cells (APC) in stimulation cultures of major histocompatibility complex class II‐restricted T cells. It was found that they induce antigen‐dependent lymphokine production by T cells and therefore could present exogenous antigens. These processing takes place intracellularly, because fixation abrogates presentation to T cells. Moreover, antigen presentation needs intracellular processing within endo‐ or lysosomes as chloroquine‐treatment prevents T cell activation. Titration of APC numbers revealed that contaminating APC most likely did not account for antigen‐specific T cell activation by DC. No evidence was found for release of antigenic peptides or for partial antigen processing possibly done by cell surface located enzymes on DC. In conclusion, these results indicate that freshly enriched DC are able to process antigens similarly to other APC.
AB - The antibody 4F7 was reported to recognize an epitope expressed on dendritic cells (DC) from various tissues. To study the ability of splenic 4F7+ dendritic cells to process antigen for presentation to CD4+ T cells, DC were enriched using a separation procedure avoiding overnight culture which could lead to an altered phenotype. These DC were used as antigen‐presenting cells (APC) in stimulation cultures of major histocompatibility complex class II‐restricted T cells. It was found that they induce antigen‐dependent lymphokine production by T cells and therefore could present exogenous antigens. These processing takes place intracellularly, because fixation abrogates presentation to T cells. Moreover, antigen presentation needs intracellular processing within endo‐ or lysosomes as chloroquine‐treatment prevents T cell activation. Titration of APC numbers revealed that contaminating APC most likely did not account for antigen‐specific T cell activation by DC. No evidence was found for release of antigenic peptides or for partial antigen processing possibly done by cell surface located enzymes on DC. In conclusion, these results indicate that freshly enriched DC are able to process antigens similarly to other APC.
KW - Antigen presentation
KW - Antigen processing
KW - CD4 T cells
KW - Dendritic cell
KW - Mice
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U2 - 10.1002/eji.1830241238
DO - 10.1002/eji.1830241238
M3 - Article
C2 - 7805745
AN - SCOPUS:0028589138
SN - 0014-2980
VL - 24
SP - 3170
EP - 3174
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 12
ER -