Frequency- and duration-dependent effects of cyclic pressure on select bone cell functions

Jiro Nagatomi, Bernard P. Arulanandam, Dennis W. Metzger, Alain Meunier, Rena Bizios

Research output: Contribution to journalArticlepeer-review

76 Scopus citations


The present study demonstrated unique correlations between characteristic parameters of mechanical loading and osteoblast functions. Specifically, osteoblast proliferation was dependent on the frequency and on the duration of the applied cyclic pressure stimulus: decreased cell proliferation was only observed when these cells were exposed to cyclic pressure at 1.0-Hz (but not at 0.25-Hz) frequency for 1 h (but not for 20 min) daily for 5 days. In contrast, endothelial cells were not responsive to cyclic pressure, whereas fibroblast proliferation increased under similar test conditions. Most important, cyclic pressure affected various osteoblast genes differently: exposure of osteoblasts to cyclic pressure (at 1.0-Hz frequency for 1 h daily) resulted in enhanced transcription and translation of alkaline phosphatase after 5 days; the same mechanical stimulus, however, did not affect osteopontin mRNA expression during the same time periods. These findings provide cellular and molecular level information, which is not only important in elucidating the correlation between mechanical loading and bone homeostasis, but can be useful in development of new technology in skeletal tissue engineering.

Original languageEnglish (US)
Pages (from-to)717-728
Number of pages12
JournalTissue Engineering
Issue number6
StatePublished - Dec 1 2001
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Cell Biology


Dive into the research topics of 'Frequency- and duration-dependent effects of cyclic pressure on select bone cell functions'. Together they form a unique fingerprint.

Cite this