Free Radical Scavenging, Antitumor and Anticarcinogenic Activity of Gossypin

B. H. Babu, H. N. Jayram, M. G. Nair, K. B. Ajaikumar, Jose Padikkala

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

Antioxidant, antitumor and anticarcinogenic activity of gossypin (3,5,8,3′,4′-pentahydroxy-7-O glucosyl flavone) was carried out. The compound needed for 50% inhibition of superoxide, hydroxyl and nitric oxide radicals was 3 μg/ml, 41 μg/ml and 12 μg/ml, respectively. Gossypin also impart 50% inhibition at concentrations 37 μg/ml and 43 μg/ml, respectively for in vitro lipid peroxidation. The compound shows IC 50 of 30 μM, 42.5 μM and 45.1 μM concentrations in L 929, HT 29 and K 562 cell lines in 72 hrs MTT assay. The compound shows a zone of inhibition of 8 mm in topo I and topo II inhibition assay in Saccharomyces ceriviseae mutant cultures. It reduces the tumor burden in solid tumor harboring animals (p<0.001) and effectively inhibits the formation of new blood vessels on tumor mass. 20 mg/kg b.wt of gossypin increase the life span of ascites tumor harboring animals (100%) and (164.7%) respectively by oral and intraperitoneal administration of the drug. Gossypin reduced the incidence of papilloma formation and papilloma/mouse in DMBA/croton oil induced skin papilloma.

Original languageEnglish (US)
Pages (from-to)581-589
Number of pages9
JournalJournal of Experimental and Clinical Cancer Research
Volume22
Issue number4
StatePublished - Dec 2003
Externally publishedYes

Keywords

  • Anticarcinogenic activity
  • Antioxidant
  • Antitumour
  • Gossypin
  • MTT assay
  • Topoisomerase I and II inhibition

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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