Fractionation of mouse epidermal chromatin components

T. J. Slaga, S. B. Das, J. M. Rice, S. Thompson

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Epidermis from shaved CD1 mice (7 to 9 wk old) in the resting phase of the hair cycle was isolated by first employing a depilatory agent followed by a mild heat treatment of the skin. A method for isolating mouse epidermal chromatin and the subsequent isolation of histones and nonhistone chromosomal proteins is described. The relative proportions of the chromatin components and the ultraviolet absorption spectra were similar to those of chromatin from mouse or rat liver. Electrophoresis of the histones from epidermal chromatin in acidic polyacrylamide gels containing urea revealed a pattern of histones qualitatively similar to that obtained from mouse or rat liver chromatin. The nonhistone chromosomal proteins of mouse epidermis were analyzed on high resolution sodium dodecyl sulfate (SDS) polyacrylamide gels. The preparation of mouse nonhistone chromosomal proteins revealed approximately 30 bands. A comparison of the SDS gel patterns of nonhistone chromosomal proteins from mouse epidermis, mouse liver and rat liver revealed some similarities especially in the high and low molecular weight regions and several differences in the high and middle molecular weight regions.

Original languageEnglish (US)
Pages (from-to)343-349
Number of pages7
JournalJournal of Investigative Dermatology
Volume63
Issue number4
DOIs
StatePublished - 1974

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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