TY - JOUR
T1 - Fractionation of human lymphocytes with plant lectins. II. Lens culinaris lectin and wheat germ agglutinin identify distinct lymphocyte subclasses
AU - Boldt, D. H.
AU - Lyons, R. D.
PY - 1979/12/1
Y1 - 1979/12/1
N2 - We have fractionated human peripheral blood lymphocytes (PBL) into distinct subclasses based upon differential adherence to the plant lectins wheat germ agglutinin (WGA) and Lens culinaris lectin (lentil-PHA), derivatized to gelatin surfaces in plastic tubes and Petri dishes. Fifteen to forty percent of PBL were adherent to WGA-derivatized gelatin. Binding studies with 125I-WGA showed that, compared to unfractionated PBL which bound 31.9 x 106 WGA molecules/cell, adherent cells bound more (44.1 x 106 molecules/cell), and nonadherent cells bound less (20.4 x 106 molecules/cell) WGA. By contrast, there were no differences among these groups for binding of other plant lectins. When lentil-PHA-derivatized gelatin was used, 25 to 50% of PBL were adherent, and, as previously reported, adherent cells bound approximately 4 times as many lentil-PHA molecules as nonadherent cells, whereas there were no differences in binding of other lectins. Sequential fractionation studies indicated that the WGA- and lentil-PHA-selected subclasses were separate groups. The percentage of T and B lymphocytes and macrophages did not differ among the subclasses. WGA-selected and lentil-PHA-selected lymphocyte subpopulations responded differently to stimulation in vitro by a panel of plant mitogens. Human PBL contain a spectrum of cell subclasses with different lectin-binding capacities. These subclasses are functionally unique.
AB - We have fractionated human peripheral blood lymphocytes (PBL) into distinct subclasses based upon differential adherence to the plant lectins wheat germ agglutinin (WGA) and Lens culinaris lectin (lentil-PHA), derivatized to gelatin surfaces in plastic tubes and Petri dishes. Fifteen to forty percent of PBL were adherent to WGA-derivatized gelatin. Binding studies with 125I-WGA showed that, compared to unfractionated PBL which bound 31.9 x 106 WGA molecules/cell, adherent cells bound more (44.1 x 106 molecules/cell), and nonadherent cells bound less (20.4 x 106 molecules/cell) WGA. By contrast, there were no differences among these groups for binding of other plant lectins. When lentil-PHA-derivatized gelatin was used, 25 to 50% of PBL were adherent, and, as previously reported, adherent cells bound approximately 4 times as many lentil-PHA molecules as nonadherent cells, whereas there were no differences in binding of other lectins. Sequential fractionation studies indicated that the WGA- and lentil-PHA-selected subclasses were separate groups. The percentage of T and B lymphocytes and macrophages did not differ among the subclasses. WGA-selected and lentil-PHA-selected lymphocyte subpopulations responded differently to stimulation in vitro by a panel of plant mitogens. Human PBL contain a spectrum of cell subclasses with different lectin-binding capacities. These subclasses are functionally unique.
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M3 - Article
C2 - 379219
AN - SCOPUS:0018758899
VL - 123
SP - 808
EP - 816
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 2
ER -