Fecal neutral steroids were fractionated by high performance liquid chromatography (HPLC) into three major fractions: 5β-H, 3-keto steroids; 5β-H, 3β-hydroxy steroids; and 5α-H and Δ5-3β-hydroxy steroids. This separation was achieved in about 10 minutes, with greater than 97% recovery of standards in each fraction. Gas-liquid chromatographic quantitation of fecal steroids fractionated by either HPLC or thin-layer chromatography gave nearly identical results. A method using both C18 reverse phase and silica HPLC to purify radiolabeled sterols is also described.
|Original language||English (US)|
|Number of pages||5|
|Journal||Journal of lipid research|
|State||Published - 1985|
ASJC Scopus subject areas
- Cell Biology