Fractionation of fecal neutral steroids by high performance liquid chromatography

E. M. Jackson, C. A. Kloss, S. T. Weintraub, G. E. Mott

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Fecal neutral steroids were fractionated by high performance liquid chromatography (HPLC) into three major fractions: 5β-H, 3-keto steroids; 5β-H, 3β-hydroxy steroids; and 5α-H and Δ5-3β-hydroxy steroids. This separation was achieved in about 10 minutes, with greater than 97% recovery of standards in each fraction. Gas-liquid chromatographic quantitation of fecal steroids fractionated by either HPLC or thin-layer chromatography gave nearly identical results. A method using both C18 reverse phase and silica HPLC to purify radiolabeled sterols is also described.

Original languageEnglish (US)
Pages (from-to)893-897
Number of pages5
JournalJournal of lipid research
Issue number7
StatePublished - 1985
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology


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