Fractionation and immunologic assessment of KCl-extracted cardiac antigens in coxsackievirus B3 virus-induced myocarditis

R. E. Paque, D. C. Straus, T. J. Nealon, C. J. Gauntt

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Hypertonic salt extracts prepared from the heart tissues of adolescent CD-1 mice were fractionated on Sephadex G-100 columns. Two separate fractions were obtained. Fraction I, containing the antigenic immunoreactive activity, was able to inhibit the migration of CVB3-PPD immune mouse peritoneal exudate cells (IMPEC) as well as PEC from mice infected with CVB3 virus alone. Fraction II did not have antigenic activity as assessed by the agarose droplet cell migration inhibition assay. As controls, Fraction I prepared from the livers or spleens of CVB3-infected CD-1 mice was unable to inhibit the migration of CVB3 IMPEC. Unimmunized or 'normal' mouse peritoneal exudate cells (NMPEC) were not inhibited by Fractin I. Antibodies prepared against Fractions I and II were unable to neutralize CVB3(m) virus in the plaque reduction test, and polyacrylamide gel analysis revealed multiple bands in 10% SDS gels.

Original languageEnglish (US)
Pages (from-to)358-364
Number of pages7
JournalJournal of Immunology
Volume123
Issue number1
StatePublished - 1979

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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