Formation of prostacyclin-sensitive platelet aggregates in human whole blood in vitro. Part I. The occurrence of the phenomenon in healthy Male volunteers

B. Spławinska; W. Furmaga; M. Nieszpaur; W. Ziembicki; J. Kuzniar; Z. Dłuska; A. Rybak; J. Spławinski

doi:10.1080/00365518709168879

Formation of prostacyclin-sensitive platelet aggregates in human whole blood in vitro. Part I. The occurrence of the phenomenon in healthy Male volunteers

B. Spławinska, W. Furmaga, M. Nieszpaur, W. Ziembicki, J. Kuzniar, Z. Dłuska, A. Rybak, J. Spławinski

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

When unstirred citrated blood of young males was left to stand at 21 °C, the number of free platelets decreased as measured with a whole blood platelet counter. This decrease indicated spontaneous platelet aggregation (SPA) since it was accompanied by the time-dependent increase in platelet micro-aggregates and plasma beta-thromboglobulin while lactate dehydrogenase level did not change significantly. Addition of PGI₂ to whole blood increased free platelet count and decreased the number of platelet micro-aggregates. The de-aggregatory effect of PGI₂ (measured as a percentage increase in free platelet number) was correlated with the initial amount of platelet micro-aggregates. Blood storage enhanced SPA and de-aggregatory effect of prostacyclin. Immediately after blood collection de-aggregatory effect of prostacyclin was absent. Our results favour an assumption that prostacyclin-sensitive platelet aggregates in blood of young volunteers are formed in vitro rather than in vivo.

Original language	English (US)
Pages (from-to)	119-124
Number of pages	6
Journal	Scandinavian Journal of Clinical and Laboratory Investigation
Volume	47
Issue number	2
DOIs	https://doi.org/10.1080/00365518709168879
State	Published - 1987
Externally published	Yes

Keywords

ADP
Circulating platelet aggregates
Spontaneous platelet aggregation

ASJC Scopus subject areas

Clinical Biochemistry

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10.1080/00365518709168879

Cite this

Spławinska, B., Furmaga, W., Nieszpaur, M., Ziembicki, W., Kuzniar, J., Dłuska, Z., Rybak, A., & Spławinski, J. (1987). Formation of prostacyclin-sensitive platelet aggregates in human whole blood in vitro. Part I. The occurrence of the phenomenon in healthy Male volunteers. Scandinavian Journal of Clinical and Laboratory Investigation, 47(2), 119-124. https://doi.org/10.1080/00365518709168879

Formation of prostacyclin-sensitive platelet aggregates in human whole blood in vitro. Part I. The occurrence of the phenomenon in healthy Male volunteers. / Spławinska, B.; Furmaga, W.; Nieszpaur, M. et al.
In: Scandinavian Journal of Clinical and Laboratory Investigation, Vol. 47, No. 2, 1987, p. 119-124.

Research output: Contribution to journal › Article › peer-review

Spławinska, B, Furmaga, W, Nieszpaur, M, Ziembicki, W, Kuzniar, J, Dłuska, Z, Rybak, A & Spławinski, J 1987, 'Formation of prostacyclin-sensitive platelet aggregates in human whole blood in vitro. Part I. The occurrence of the phenomenon in healthy Male volunteers', Scandinavian Journal of Clinical and Laboratory Investigation, vol. 47, no. 2, pp. 119-124. https://doi.org/10.1080/00365518709168879

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abstract = "When unstirred citrated blood of young males was left to stand at 21 °C, the number of free platelets decreased as measured with a whole blood platelet counter. This decrease indicated spontaneous platelet aggregation (SPA) since it was accompanied by the time-dependent increase in platelet micro-aggregates and plasma beta-thromboglobulin while lactate dehydrogenase level did not change significantly. Addition of PGI2 to whole blood increased free platelet count and decreased the number of platelet micro-aggregates. The de-aggregatory effect of PGI2 (measured as a percentage increase in free platelet number) was correlated with the initial amount of platelet micro-aggregates. Blood storage enhanced SPA and de-aggregatory effect of prostacyclin. Immediately after blood collection de-aggregatory effect of prostacyclin was absent. Our results favour an assumption that prostacyclin-sensitive platelet aggregates in blood of young volunteers are formed in vitro rather than in vivo.",

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AU - Spławinska, B.

AU - Furmaga, W.

AU - Nieszpaur, M.

AU - Ziembicki, W.

AU - Kuzniar, J.

AU - Dłuska, Z.

AU - Rybak, A.

AU - Spławinski, J.

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N2 - When unstirred citrated blood of young males was left to stand at 21 °C, the number of free platelets decreased as measured with a whole blood platelet counter. This decrease indicated spontaneous platelet aggregation (SPA) since it was accompanied by the time-dependent increase in platelet micro-aggregates and plasma beta-thromboglobulin while lactate dehydrogenase level did not change significantly. Addition of PGI2 to whole blood increased free platelet count and decreased the number of platelet micro-aggregates. The de-aggregatory effect of PGI2 (measured as a percentage increase in free platelet number) was correlated with the initial amount of platelet micro-aggregates. Blood storage enhanced SPA and de-aggregatory effect of prostacyclin. Immediately after blood collection de-aggregatory effect of prostacyclin was absent. Our results favour an assumption that prostacyclin-sensitive platelet aggregates in blood of young volunteers are formed in vitro rather than in vivo.

AB - When unstirred citrated blood of young males was left to stand at 21 °C, the number of free platelets decreased as measured with a whole blood platelet counter. This decrease indicated spontaneous platelet aggregation (SPA) since it was accompanied by the time-dependent increase in platelet micro-aggregates and plasma beta-thromboglobulin while lactate dehydrogenase level did not change significantly. Addition of PGI2 to whole blood increased free platelet count and decreased the number of platelet micro-aggregates. The de-aggregatory effect of PGI2 (measured as a percentage increase in free platelet number) was correlated with the initial amount of platelet micro-aggregates. Blood storage enhanced SPA and de-aggregatory effect of prostacyclin. Immediately after blood collection de-aggregatory effect of prostacyclin was absent. Our results favour an assumption that prostacyclin-sensitive platelet aggregates in blood of young volunteers are formed in vitro rather than in vivo.

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KW - Circulating platelet aggregates

KW - Spontaneous platelet aggregation

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Formation of prostacyclin-sensitive platelet aggregates in human whole blood in vitro. Part I. The occurrence of the phenomenon in healthy Male volunteers

Abstract

Keywords

ASJC Scopus subject areas

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