A procedure for the preparation of a ternary complex, guanylyl-5′-methylene diphosphonate-EF-Tu-Phe-tRNA is described. This complex is sufficiently stable to persist purification by gel filtration chromatography. Such a complex can bind to ribosomes in the presence of poly U.
|Original language||English (US)|
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Apr 21 1975|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology