Formation of aminoacyl-tRNA-guanylyl-5′-methylene diphosphonate-elongation factor complex

John C. Lee, Mary C. Roach

Research output: Contribution to journalArticlepeer-review

Abstract

A procedure for the preparation of a ternary complex, guanylyl-5′-methylene diphosphonate-EF-Tu-Phe-tRNA is described. This complex is sufficiently stable to persist purification by gel filtration chromatography. Such a complex can bind to ribosomes in the presence of poly U.

Original languageEnglish (US)
Pages (from-to)864-869
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume63
Issue number4
DOIs
StatePublished - Apr 21 1975

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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