Formation of aminoacyl-tRNA-guanylyl-5′-methylene diphosphonate-elongation factor complex

John C. Lee; Mary C. Roach

doi:10.1016/0006-291X(75)90647-6

Formation of aminoacyl-tRNA-guanylyl-5′-methylene diphosphonate-elongation factor complex

John C. Lee, Mary C. Roach

Department of Biochemistry & Structural Biology

Research output: Contribution to journal › Article › peer-review

Abstract

A procedure for the preparation of a ternary complex, guanylyl-5′-methylene diphosphonate-EF-Tu-Phe-tRNA is described. This complex is sufficiently stable to persist purification by gel filtration chromatography. Such a complex can bind to ribosomes in the presence of poly U.

Original language	English (US)
Pages (from-to)	864-869
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	63
Issue number	4
DOIs	https://doi.org/10.1016/0006-291X(75)90647-6
State	Published - Apr 21 1975

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(75)90647-6

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title = "Formation of aminoacyl-tRNA-guanylyl-5′-methylene diphosphonate-elongation factor complex",

abstract = "A procedure for the preparation of a ternary complex, guanylyl-5′-methylene diphosphonate-EF-Tu-Phe-tRNA is described. This complex is sufficiently stable to persist purification by gel filtration chromatography. Such a complex can bind to ribosomes in the presence of poly U.",

author = "Lee, {John C.} and Roach, {Mary C.}",

note = "Funding Information: The authors are indebted to Dr. Joanne Ravel for supplying us initially with samples of purified elongation factors. This work was supported in part by a grant from the Robert A. Welch Foundation (AQ 521).",

year = "1975",

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AU - Roach, Mary C.

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Formation of aminoacyl-tRNA-guanylyl-5′-methylene diphosphonate-elongation factor complex

Abstract

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