Abstract
The influence of food restriction (FR) on the drug-inducible capacity of liver microsomal cytochrome P-450s IA1,IA2 and IIB1 and IIB2 was studied in 20-month-old male Fischer-344 rats. ELISA and Western Blotting revealed that the induction of the cytochrome P-450-IA1/IA2 and P-450-IIB1/IIB2 enzymes was considerably higher in the liver microsomes of FR rats than in their ad libitum (AL) fed counterparts. In order to determine whether the higher P-450 enzyme levels in FR rats were a reflection of an increased synthesis rate or a stabilization of these enzymes, hybridization studies were performed with a cDNA probe for P-450-IIB1/1IIB2. These studies show markedly higher levels of P-450-IIB1/IIB2 mRNAs in the livers of FR rats as compared to AL animals. These results suggest that it is possible to prevent the age-dependent loss of drug-induced cytochrome P-450s by 40% dietary restriction which suggest FR may improve the drug-metabolizing capacity during aging.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 725-730 |
| Number of pages | 6 |
| Journal | Biochemistry International |
| Volume | 20 |
| Issue number | 4 |
| State | Published - 1990 |
ASJC Scopus subject areas
- Biochemistry