Food restriction modulates β-adrenergic-sensitive adenylate cyclase in rat liver during aging

M. S. Katz

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Adenylate cyclase activities were studied in rat liver during post-maturational aging of male Fischer 344 rats fed ad libitum or restricted to 60% of the ad libitum intake. Catecholamine (10-5 M epinephrine, isoproterenol, or norepinephrine)-stimulated adenylate cyclase activity increased by 200-300% between 6 and 24-27 mo of age in ad libitum-fed rats, whereas in food-restricted rats catecholamine response increased by only 58-84% between 6 and 30 mo. In ad libitum-fed rats, glucagon (10-5 M)-stimulated enzyme activity also increased by 40% between 6 and 12 mo and in restricted rats a similar age-related increase was delayed until 18 mo. β-Adrenergic receptor density (B(max), determined from saturation binding of the β-adrenergic antagonist l-[125I]iodopindolol) increased by 50% between 6 and 24 mo in livers from ad libitum-fed but not food-restricted rats and showed a highly significant correlation with maximal isoproterenol-stimulated adenylate cyclase activity over the post-maturational life span. Age-related increases in unstimulated (basal) adenylate cyclase activity and nonreceptor-mediated enzyme activation (by guanine nucleotides, fluoride, and to a lesser extent forskolin) were retarded by food restriction. The results demonstrate that food restriction diminishes a marked age-related increase in β-adrenergic-sensitive adenylate cyclase activity of rat liver. Alterations of adrenergic-responsive adenylate cyclase with age and the modulatory effects of food restriction appear to be mediated by changes in both receptor and nonreceptor components of adenylate cyclase.

Original languageEnglish (US)
Pages (from-to)17/1
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume254
Issue number1
StatePublished - 1988

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

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